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Anti-MAP2K3 / MEK3 / MKK3 Antibody (clone 6B12) LS-C174452

Catalog Size Price
LS-C174452-100 100 µl (1 mg/ml) Unavailable

Most Popular MAP2K3 / MEK3 / MKK3 Antibodies

Anti-MAP2K3 / MEK3 / MKK3 Antibody (clone 1D10) IHC-plus™ LS-B5521
Mouse Monoclonal [clone 1D10] (IgG1,k) to Human MAP2K3 / MEK3 / MKK3
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-MAP2K3 / MEK3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5521 concentration 5 ug/ml.
Anti-MAP2K3 / MEK3 / MKK3 Antibody (clone 2A7) LS-C337935
Mouse Monoclonal [clone 2A7] (IgG2b) to Human MAP2K3 / MEK3 / MKK3
Human, Monkey, Mouse, Rat, Dog
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
IHC of paraffin-embedded Human pancreas tissue using anti-MAP2K3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Anti-MAP2K3 / MEK3 / MKK3 Antibody (clone 6B11) LS-C337938
Mouse Monoclonal [clone 6B11] (IgG2b) to Human MAP2K3 / MEK3 / MKK3
Human, Monkey, Mouse, Rat, Dog
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
IHC of paraffin-embedded Human pancreas tissue using anti-MAP2K3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Anti-MAP2K3 / MEK3 / MKK3 Antibody (phospho-Thr222) LS-C368409
Rabbit Polyclonal to Human MAP2K3 / MEK3 / MKK3
Human, Mouse, Zebrafish
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of MKK3 (pT222) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-MAP2K3 / MEK3 / MKK3 Antibody (clone 2E12D11) LS-C669433
Mouse Monoclonal [clone 2E12D11] (IgG1) to Human MAP2K3 / MEK3 / MKK3
Human
IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated

100% Guaranteed
Mouse Monoclonal [clone 6B12] (IgG2b) to Human MAP2K3 / MEK3 / MKK3
Human, Monkey, Mouse, Rat, Dog
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human MAP2K3 / MEK3 / MKK3
Mouse
Human, Monkey, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [6B12]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:200 - 1:2000)

Specificity and Use

Full length human recombinant protein of human MAP2K3(NP_659731) produced in HEK293T cell.
Human MEK3 / MKK3

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About MAP2K3 / MEK3 / MKK3

P46734 NM_002756 NP_002747.2

MAP2K3 Antibody, MAPKK 3 Antibody, MAPKK3 Antibody, PRKMK3 Antibody, SAPK kinase 2 Antibody, SAPKK-2 Antibody, SAPKK2 Antibody, SKK2 Antibody, MAP kinase kinase 3 Antibody, MEK 3 Antibody, MEK3 Antibody, MKK3 Antibody, MAPK/ERK kinase 3 Antibody

MAP2K3 / MEK3 / MKK3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter.

Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-MAP2K3 mouse monoclonal antibody.
IHC of paraffin-embedded Human pancreas tissue using anti-MAP2K3 mouse monoclonal antibody.

Immunofluorescence

Anti-MAP2K3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K3.
Anti-MAP2K3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K3.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K3.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-MAP2K3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot of extracts (35 ug) from 9 different cell lines by using g anti-MAP2K3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Requested From: 
Date Requested: 4/24/2018

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