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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAP2K2 / MKK2 / MEK2 Antibody (clone 8G6) LS‑C114570
MEK2 antibody LS-C114570 is an unconjugated mouse monoclonal antibody to human MEK2 (MAP2K2 / MKK2). Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C114570-100
100 µl
Unavailable

Popular MAP2K2 / MKK2 / MEK2 Products

Anti-MAP2K2 / MEK2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1727 concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
IHC of paraffin-embedded pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
IHC of paraffin-embedded Human liver tissue using anti-MAP2K2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Species: Human, Monkey, Mouse, Rat, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

MEK2 antibody LS-C114570 is an unconjugated mouse monoclonal antibody to human MEK2 (MAP2K2 / MKK2). Validated for Flow, IHC and WB.
About MAP2K2 / MKK2 / MEK2
MAP2K2 / MKK2 / MEK2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. P36507 NM_030662 NP_109587.1

MAP2K2 Antibody, ERK activator kinase 2 Antibody, MAP kinase kinase 2 Antibody, MAPK/ERK kinase 2 Antibody, MAPKK 2 Antibody, MKK2 Antibody, MEK 2 Antibody, MEK2 Antibody, MAPKK2 Antibody, PRKMK2 Antibody


Specifications

Target
Human MAP2K2 / MKK2 / MEK2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [8G6]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Protein expressed in 293T cell transfected with human MAP2K2 expression vector.
Specificity
Human MKK2 / MEK2
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded lung tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded lung tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of bladder tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of bladder tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded lung tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded lung tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of bladder tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of bladder tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded lung tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded lung tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of bladder tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of bladder tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of kidney tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded lung tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded lung tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of bladder tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of bladder tissue using anti-MAP2K2 mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K2 antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/23/2018

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