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Anti-MAP2K2 / MKK2 / MEK2 Antibody (aa262-292) LS-C99768

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MAP2K2 / MKK2 / MEK2 Antibodies

Anti-MAP2K2 / MKK2 / MEK2 Antibody (phospho-Thr394) IHC-plus™ LS-B1727
Rabbit Polyclonal (IgG) to Human MAP2K2 / MKK2 / MEK2
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MAP2K2 / MKK2 / MEK2 Antibody (N-Terminus) IHC-plus™ LS-B4671
Rabbit Polyclonal (IgG) to Human MAP2K2 / MKK2 / MEK2
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human MAP2K2 / MKK2 / MEK2
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human MAP2K2 / MKK2 / MEK2
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

MAP2K2 / MKK2 / MEK2 antibody was raised against kLH-conjugated synthetic peptide between amino acids 262-292 from internal region of human MEK2 (MAP2K2).
LS-E9395 - Lyophilized - 100 µg - $145.00
aa262-292

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About MAP2K2 / MKK2 / MEK2

P36507 NM_030662 NP_109587.1

MAP2K2 Antibody, ERK activator kinase 2 Antibody, MAP kinase kinase 2 Antibody, MAPK/ERK kinase 2 Antibody, MAPKK 2 Antibody, MKK2 Antibody, MEK 2 Antibody, MEK2 Antibody, MAPKK2 Antibody, PRKMK2 Antibody

MAP2K2 / MKK2 / MEK2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence

Immunofluorescence
Fluorescent image of U251 cell stained with MEK2 (MAP2K2) Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). MEK2 immunoreactivity is localized to Cytoplasm significantly.

Western blot

Western blot
MAP2K2 Antibody (A278) western blot of U937 cell line lysates (35 ug/lane). The MAP2K2 antibody detected the MAP2K2 protein (arrow).

Western blot

Western blot
The anti-MAP2K2 antibody is used in Western blot to detect MAP2K2 in mouse liver tissue lysate.

Western blot

Western blot
Western blot of MAP2K2 (arrow) using MAP2K2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2) (Origene Technologies).
 

Requested From: United States
Date Requested: 12/7/2016

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