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Anti-MAP2K1 / MKK1 / MEK1 Antibody (clone 3C1) LS-C174664

Catalog	Size	Price
LS-C174664-100	100 µl (1 mg/ml)	Unavailable

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Antibody:	Rabbit Monoclonal (IgG) to Human MAP2K1 / MKK1 / MEK1
Reactivity:	Human, Monkey, Mouse, Rat
Application:	Western blot, ELISA
Format:	Unconjugated

Anti-MAP2K1 / MKK1 / MEK1 Antibody (N-Terminus) IHC-plus™ LS-B3271

Antibody:	Rabbit Polyclonal to Human MAP2K1 / MKK1 / MEK1
Reactivity:	Human, Mouse, Rat, Xenopus
Application:	IHC - Paraffin, Immunofluorescence, Western blot
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Anti-MAP2K1 / MKK1 / MEK1 Antibody (Internal) LS-C356181

Antibody:	Rabbit Polyclonal to Human MAP2K1 / MKK1 / MEK1
Reactivity:	Human, Monkey, Mouse, Rat, Bovine, Pig, Rabbit
Application:	IHC - Paraffin, Western blot
Format:	Unconjugated

Anti-MAP2K1 / MKK1 / MEK1 Antibody (phospho-Thr292) LS-C380779

Antibody:	Rabbit Polyclonal (IgG) to Human MAP2K1 / MKK1 / MEK1
Reactivity:	Human, Mouse, Rat
Application:	IHC, Immunofluorescence, Western blot, ELISA
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Anti-MAP2K1 / MKK1 / MEK1 Antibody LS-C497659

Antibody:	Rabbit Monoclonal (IgG) to Human MAP2K1 / MKK1 / MEK1
Reactivity:	Human
Application:	IHC, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Format:	Unconjugated

Antibody:	Mouse Monoclonal [clone 3C1] (IgG2a) to Human MAP2K1 / MKK1 / MEK1
Reactivity:	Human, Monkey, Mouse, Dog
Application:	IHC - Paraffin, Immunofluorescence, Western blot
Format:	Unconjugated

Details

Target:	Human MAP2K1 / MKK1 / MEK1
Host:	Mouse
Reactivity:	Human, Monkey, Mouse, Dog (tested or 100% immunogen sequence identity)
Clonality:	IgG2a Monoclonal [3C1]
Conjugations:	Unconjugated
Purification:	Protein A/G purified
Modifications:	Unmodified

Applications

Approved:

IHC - Paraffin (1:150)
Immunofluorescence (1:100)
Western blot (1:200 - 1:4000)

Specificity and Use

Immunogen:	MAP2K1 / MKK1 / MEK1 antibody was raised against full length human recombinant protein of human MAP2K1(NP_002746) produced in HEK293T cell.
Specificity:	Human MEK1

Packaging

Presentation:	PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage:	Store at -20°C. Minimize freezing and thawing.
Restrictions:	For research use only.

About MAP2K1 / MKK1 / MEK1

Uniprot: Q02750 NCBI: NM_002755 NP_002746.1

Synonyms: MAP2K1 Antibody, ERK activator kinase 1 Antibody, MAPK/ERK kinase 1 Antibody, MAPKK 1 Antibody, MKK1 Antibody, PRKMK1 Antibody, MEK 1 Antibody, MAP kinase kinase 1 Antibody, MAPKK1 Antibody, MEK1 Antibody

Description: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2.

(More About MAP2K1 / MKK1 / MEK1)

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Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-MAP2K1 mouse monoclonal antibody.

Immunofluorescence

Anti-MAP2K1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAP2K1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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Date Requested: 6/22/2017

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