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Anti-MAP2K1 / MKK1 / MEK1 Antibody (clone 3C1) LS-C174664

Catalog	Size	Price
LS-C174664-100	100 µl (1 mg/ml)	Unavailable

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225 MAP2K1 / MKK1 / MEK1 Antibodies

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Anti-MAP2K1 / MKK1 / MEK1 Antibody (phospho-Ser218/Ser222) LS-C25552

Antibody:	Mouse Monoclonal (IgG1) to Human MAP2K1 / MKK1 / MEK1
Reactivity:	Human, Mouse, Dog
Application:	Western blot, ELISA
Format:	Unconjugated

Anti-MAP2K1 / MKK1 / MEK1 Antibody LS-C25563

Antibody:	Mouse Monoclonal (IgG1) to Mouse MAP2K1 / MKK1 / MEK1
Reactivity:	Mouse
Application:	Western blot, Immunoprecipitation
Format:	Unconjugated

Anti-MAP2K1 / MKK1 / MEK1 Antibody (phospho-Ser218) LS-C25566

Antibody:	Rabbit Polyclonal (IgG) to Human MAP2K1 / MKK1 / MEK1
Reactivity:	Human, Mouse, Rat
Application:	Western blot
Format:	Unconjugated

Anti-MAP2K1 / MKK1 / MEK1 Antibody (N-Terminus) IHC-plus™ LS-B3271

Antibody:	Rabbit Polyclonal to Human MAP2K1 / MKK1 / MEK1
Reactivity:	Human, Mouse, Rat, Xenopus
Application:	IHC, IHC - Paraffin, Immunofluorescence, Western blot
Format:	Unconjugated

Anti-MAP2K1 / MEK1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3271 concentration 10 ug/ml.

Anti-MAP2K1 / MKK1 / MEK1 Antibody (Internal) LS-C356181

Antibody:	Rabbit Polyclonal to Human MAP2K1 / MKK1 / MEK1
Reactivity:	Human, Monkey, Mouse, Rat, Bovine, Pig, Rabbit
Application:	IHC, IHC - Paraffin, Western blot
Format:	Unconjugated

Immunohistochemical analysis of MKK1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Antibody:	Mouse Monoclonal [clone 3C1] (IgG2a) to Human MAP2K1 / MKK1 / MEK1
Reactivity:	Human, Monkey, Mouse, Dog
Application:	IHC, IHC - Paraffin, Immunofluorescence, Western blot
Format:	Unconjugated

IHC of paraffin-embedded Human pancreas tissue using anti-MAP2K1 mouse monoclonal antibody.

Anti-MAP2K1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K1.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K1.

Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAP2K1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Details

Target:	Human MAP2K1 / MKK1 / MEK1
Host:	Mouse
Reactivity:	Human, Monkey, Mouse, Dog (tested or 100% immunogen sequence identity)
Clonality:	IgG2a Monoclonal [3C1]
Conjugations:	Unconjugated
Purification:	Protein A/G purified
Modifications:	Unmodified

Applications

Approved:

IHC
IHC - Paraffin (1:150)
Immunofluorescence (1:100)
Western blot (1:200 - 1:4000)

Specificity and Use

Immunogen:	Full length human recombinant protein of human MAP2K1(NP_002746) produced in HEK293T cell.
Specificity:	Human MEK1

Packaging

Presentation:	PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage:	Store at -20°C. Avoid freeze-thaw cycles.
Restrictions:	For research use only.

About MAP2K1 / MKK1 / MEK1

Uniprot: Q02750 NCBI: NM_002755 NP_002746.1

Synonyms: MAP2K1 Antibody, ERK activator kinase 1 Antibody, MAPK/ERK kinase 1 Antibody, MAPKK 1 Antibody, MKK1 Antibody, PRKMK1 Antibody, MEK 1 Antibody, MAP kinase kinase 1 Antibody, MAPKK1 Antibody, MEK1 Antibody

Description: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2.

(More About MAP2K1 / MKK1 / MEK1)

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Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-MAP2K1 mouse monoclonal antibody.

Immunofluorescence

Anti-MAP2K1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAP2K1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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Date Requested: 4/26/2018

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