MAP2 antibody LS-C16317 is an unconjugated mouse monoclonal antibody to MAP2 from human, rat, bovine and other species. Validated for IF, IHC and WB.
Bovine, Human, Rat, Xenopus
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- IHC - Paraffin
- Western blot (1 - 2 µg/ml)
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
MAP2 antibody was raised against bovine brain microtubule associated protein 2. Epitope: aa1-151. Cellular Localization: Cytoplasmic.
Highly specific to MAP2a, b, c and shows no cross-reactivity with other MAPs, tau and tubulin. Recognizes MAP2a and MAP2b (~280kD doublet) and MAP2c (70kD). Its epitope maps in the first 151 amino acids of MAP2. Reacts with dendrites and cell bodies of neurons. Species cross-reactivity: Human, bovine, rat, Xenopus and quail.
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Western Blot: 1-2 ug/ml for 2hrs at RT. Immunohistochemistry (formalin/paraffin): 1-2 ug/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6 for 10-20 min., followed by cooling at RT for 20 min. Positive control: IMR-5 or T98G human glioblastoma cells or brain. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
10 mM PBS, pH 7.4
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.