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Anti-MAP1LC3B / LC3B Antibody (aa89-122) LS-C168823

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MAP1LC3B / LC3B Antibodies

Anti-MAP1LC3B / LC3B Antibody (aa1-100, Biotin) LS-C83716
Rabbit Polyclonal to Human MAP1LC3B / LC3B
Human, Rat, Zebrafish
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Biotin Conjugated
Immunohistochemistry Image
Anti-MAP1LC3B / LC3B Antibody (aa25-121, Biotin) LS-C83721
Rabbit Polyclonal to Human MAP1LC3B / LC3B
Human, Mouse, Rat
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Biotin Conjugated
Immunohistochemistry Image
Anti-MAP1LC3B / LC3B Antibody (N-Terminus) IHC-plus™ LS-B9650
Rabbit Polyclonal to Human MAP1LC3B / LC3B
Human, Mouse
IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human MAP1LC3B / LC3B
Human, Mouse
IHC, ICC, Immunofluorescence
Unconjugated

Details

Human MAP1LC3B / LC3B
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • ICC (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)

Specificity and Use

MAP1LC3B / LC3B antibody was raised against kLH-conjugated synthetic peptide between amino acids 89-122 from human Cleaved LC3B.
LS-E5542 - Lyophilized - 100 µg - $145.00
aa89-122
Human MAP1LC3B / LC3B

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About MAP1LC3B / LC3B

Q9GZQ8 NM_022818 NP_073729.1

MAP1LC3B Antibody, ATG8F Antibody, LC3B Antibody, MAP1A/1BLC3 Antibody, MAP1A/MAP1B LC3 B Antibody, MAP1A/MAP1B light chain 3 B Antibody, MAP1ALC3 Antibody, MAP1LC3B-a Antibody

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

Immunofluorescence

Immunofluorescence
SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4% of paraformaldehyde (right panel). Treatment with antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.

Immunofluorescence

Immunofluorescence
Time course study of mouse leukemic monocyte macrophage cells treated with U18666A, a drug that causes cholesterol and lipid storage in cells, thereby blocking fusion between late endosomes and lysosomes. Cleaved-LC3 (APG8b) antibody detected punctuate staining indicative of autophagic vacuole or phagosome structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.

Immunofluorescence

Immunofluorescence
Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
 

Requested From: United States
Date Requested: 12/8/2016

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