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Anti-MAP1LC3B / LC3B Antibody (aa1-30) LS-C165711

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

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Most Popular MAP1LC3B / LC3B Antibodies

Anti-MAP1LC3B / LC3B Antibody (aa1-100, Biotin) LS-C83716
Rabbit Polyclonal to Human MAP1LC3B / LC3B
Human, Rat, Zebrafish
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Biotin Conjugated
Immunohistochemistry Image
Anti-MAP1LC3B / LC3B Antibody (aa25-121, Biotin) LS-C83721
Rabbit Polyclonal to Human MAP1LC3B / LC3B
Human, Mouse, Rat
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Biotin Conjugated
Immunohistochemistry Image
Anti-MAP1LC3B / LC3B Antibody (N-Terminus) IHC-plus™ LS-B9650
Rabbit Polyclonal to Human MAP1LC3B / LC3B
Human, Mouse
IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (6)
Rabbit Polyclonal to Human MAP1LC3B / LC3B
Human, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human MAP1LC3B / LC3B
Rabbit
Human, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)

Specificity and Use

MAP1LC3B / LC3B antibody was raised against kLH-conjugated synthetic peptide between amino acids 1-30 from N-terminal region of human LC3.
LS-E5507 - Lyophilized - 100 µg - $145.00
aa1-30
Human MAP1LC3B / LC3B

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (6)

1
Active ras triggers death in glioblastoma cells through hyperstimulation of macropinocytosis. Overmeyer JH, Kaul A, Johnson EE, Maltese WA. Molecular cancer research : MCR. 2008 6:965-77. [PubMed:18567800] [PMC:PMC2994605]
2
Lysosomal degradation of endocytosed proteins depends on the chloride transport protein ClC-7. Wartosch L, Fuhrmann JC, Schweizer M, Stauber T, Jentsch TJ. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2009 23:4056-68. [PubMed:19661288]
3
Rab5 and class III phosphoinositide 3-kinase Vps34 are involved in hepatitis C virus NS4B-induced autophagy. Su WC, Chao TC, Huang YL, Weng SC, Jeng KS, Lai MM. Journal of virology. 2011 85:10561-71. [PubMed:21835792] [PMC:PMC3187495]
4
Caspase-6 activity in a BACHD mouse modulates steady-state levels of mutant huntingtin protein but is not necessary for production of a 586 amino acid proteolytic fragment. Gafni J, Papanikolaou T, Degiacomo F, Holcomb J, Chen S, Menalled L, Kudwa A, Fitzpatrick J, Miller S, Ramboz S, Tuunanen PI, Lehtimki KK, Yang XW, Park L, Kwak S, Howland D, Park H, Ellerby LM. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2012 32:7454-65. [PubMed:22649225] [PMC:PMC3454486]
5
Chronic autophagy is a cellular adaptation to tumor acidic pH microenvironments. Wojtkowiak JW, Rothberg JM, Kumar V, Schramm KJ, Haller E, Proemsey JB, Lloyd MC, Sloane BF, Gillies RJ. Cancer research. 2012 72:3938-47. [PubMed:22719070]
6
Induction of autophagy by Imatinib sequesters Bcr-Abl in autophagosomes and down-regulates Bcr-Abl protein. Gafni J, Papanikolaou T, Degiacomo F, Holcomb J, Chen S, Menalled L, Kudwa A, Fitzpatrick J, Miller S, Ramboz S, Tuunanen PI, Lehtimki KK, Yang XW, Park L, Kwak S, Howland D, Park H, Ellerby LM. American journal of hematology. 2013 [PubMed:23440701]
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About MAP1LC3B / LC3B

Q9GZQ8 NM_022818 NP_073729.1

MAP1LC3B Antibody, ATG8F Antibody, LC3B Antibody, MAP1A/1BLC3 Antibody, MAP1A/MAP1B LC3 B Antibody, MAP1A/MAP1B light chain 3 B Antibody, MAP1ALC3 Antibody, MAP1LC3B-a Antibody

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

Immunofluorescence

Immunofluorescence
Immunofluorescence of U251 cells, using LC3 Antibody (APG8B). U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Immunofluorescence

Immunofluorescence
Immunofluorescence of U251 cells, using LC3 Antibody (APG8B). U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Immunofluorescence

Immunofluorescence
Immunofluorescence of U251 cells, using LC3 Antibody (APG8B). U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
Western blot of anti-LC3 (APG8b) antibody in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2 ug/lane). Human LC3 (APG8b) (arrow) was detected using the purified antibody.

Western blot

Western blot
Western blot of anti-LC3 (APG8b) antibody in rat brain lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in membrane fraction (P) but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).

Western blot

Western blot
Western blot of anti-LC3 (APG8b) in untreated or treated HeLa cell lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).

Western blot

Western blot
Western blot of lysates from NIH/3T3 cell line, untreated or treated with chloroquine, using LC3 Antibody (APG8B) (upper) or Beta-actin (lower).

Western blot

Western blot
Western blot of lysates from HepG2 cell line, untreated or treated with chloroquine, using LC3 Antibody (upper) or Beta-actin (lower).

Western blot

Western blot
Western blot of lysates from HepG2, mouse NIH/3T3 cell line, untreated or treated with chloroquine, 50uM, using LC3 Antibody (APG8B) (upper) or Beta-actin (lower).

Western blot

Western blot
Western blot of lysate from mouse NIH/3T3 cell line, using LC3 Antibody (APG8B). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot

Western blot
Western blot of lysate from mouse NIH/3T3 cell line, using LC3 Antibody (APG8B). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
 

Requested From: United States
Date Requested: 12/5/2016

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