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Antibodies > MAP1LC3A / LC3A > LS-C165694
 
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Catalog Number Size Price
LS-C165694-200 200 µl (0.5 mg/ml) $393 
MAP1LC3A / LC3A Antibody - 10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center.
MAP1LC3A / LC3A Antibody - Fluorescent image of U251 cells stained LC3 (APG8) antibody.U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with LC3 (APG8) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
MAP1LC3A / LC3A Antibody - Immunofluorescence of U251 cells, using LC3 Antibody (APG8). U251 cells(right) were treated with Chloroquine (50 mu M,16h). The antibody was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
MAP1LC3A / LC3A Antibody - Immunofluorescence of U251 cells, using LC3 Antibody (APG8). U251 cells(right) were treated with Chloroquine (50 mu M,16h). The antibody was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
MAP1LC3A / LC3A Antibody - Western blot of anti-LC3 antibody HeLa cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.
MAP1LC3A / LC3A Antibody - Western blot of anti-LC3 antibody at 8 ug/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).
MAP1LC3A / LC3A Antibody - 10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center.
MAP1LC3A / LC3A Antibody - Fluorescent image of U251 cells stained LC3 (APG8) antibody.U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with LC3 (APG8) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
MAP1LC3A / LC3A Antibody - Immunofluorescence of U251 cells, using LC3 Antibody (APG8). U251 cells(right) were treated with Chloroquine (50 mu M,16h). The antibody was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
MAP1LC3A / LC3A Antibody - Immunofluorescence of U251 cells, using LC3 Antibody (APG8). U251 cells(right) were treated with Chloroquine (50 mu M,16h). The antibody was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
MAP1LC3A / LC3A Antibody - Western blot of anti-LC3 antibody HeLa cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.
MAP1LC3A / LC3A Antibody - Western blot of anti-LC3 antibody at 8 ug/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).
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Monoclonal Mouse anti‑Human MAP1LC3A / LC3A Antibody (IHC, IF, WB) LS‑C165694

Monoclonal Mouse anti‑Human MAP1LC3A / LC3A Antibody (IHC, IF, WB) LS‑C165694

(4.1) Rating
Submit Review (2) Publication(s) LSBio Guarantee
Antibody:
MAP1LC3A / LC3A Mouse anti-Human Monoclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C165694-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MAP1LC3A / LC3A Mouse anti-Human Monoclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
LC3A antibody LS-C165694 is an unconjugated mouse monoclonal antibody to LC3A (MAP1LC3A) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Cited in 2 publications.
(2) Publication(s)
Target
Human MAP1LC3A / LC3A
Synonyms
MAP1LC3A | ATG8E | LC3 | LC3A | MAP1BLC3 | MAP1A/1B light chain 3 A | MAP1A/MAP1B LC3 A | MAP1A/MAP1B light chain 3 A | MAP1ALC3
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:100)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAP1LC3A / LC3A
Q9H492 NM_032514 NP_115903.1

LSBio Ratings

MAP1LC3A / LC3A Antibody for IHC, IF/Immunofluorescence, WB/Western LS-C165694 has an LSBio Rating of
(4.1) Rating
Publications (4.1)

Publications (2)

Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis. Song D, Lee FS. The Journal of biological chemistry. 2011 286:15797-805.
PubMed: 21367862 PMC: PMC3091189
Mitochondria-lysosome membrane contacts are defective in GDAP1-related Charcot-Marie-Tooth disease. Lara Cantarero , Elena Juárez-Escoto , Azahara Civera-Tregón , María Rodríguez-Sanz, Mónica Roldán, Raúl Benítez , Janet Hoenicka , Francesc Palau. The Breast : official journal of the European Society of Mastology. 2021 Jan;29:3589-3605.
Full Text Article PubMed: 33372681 PMC: PMC7823109
Submit a Publication for LS-C165694

Customer Reviews (0)

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MAP1LC3A / LC3A ELISA Kit
Mouse MAP1LC3A / LC3A (Sandwich ELISA) ELISA Kit - LS-F35934
Reactivity: Mouse
Range: 78-5000 pg/ml
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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