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Anti-MAP1LC3A / LC3A Antibody LS-C165694

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular MAP1LC3A / LC3A Antibodies

Anti-MAP1LC3A / LC3A Antibody (aa97-106) IHC-plus™ LS-B5129
Goat Polyclonal to Human MAP1LC3A / LC3A
Human, Monkey, Mouse, Rat, Bovine, Hamster, Pig, Xenopus
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-MAP1LC3A / LC3A Antibody LS-C165694
Mouse Monoclonal (IgG1,k) to Human MAP1LC3A / LC3A
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal (IgG1,k) to Human MAP1LC3A / LC3A
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated

Details

Human MAP1LC3A / LC3A
Mouse
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC (1:50 - 1:100)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)

Specificity and Use

MAP1LC3A / LC3A antibody was raised against full length recombinant protein of human LC3 (APG8).
Human LC3

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (1)

1
Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis. Song D, Lee FS. The Journal of biological chemistry. 2011 286:15797-805. [PubMed:21367862] [PMC:PMC3091189]

About MAP1LC3A / LC3A

Q9H492 NM_032514 NP_115903.1

MAP1LC3A Antibody, ATG8E Antibody, LC3 Antibody, LC3A Antibody, MAP1BLC3 Antibody, MAP1A/1B light chain 3 A Antibody, MAP1A/MAP1B LC3 A Antibody, MAP1A/MAP1B light chain 3 A Antibody, MAP1ALC3 Antibody

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

Immunohistochemistry

Immunohistochemistry
10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center.

Immunofluorescence

Immunofluorescence
Immunofluorescence of U251 cells, using LC3 Antibody (APG8). U251 cells(right) were treated with Chloroquine (50 mu M,16h). The antibody was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Immunofluorescence

Immunofluorescence
Fluorescent image of U251 cells stained LC3 (APG8) antibody.U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with LC3 (APG8) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Immunofluorescence

Immunofluorescence
Immunofluorescence of U251 cells, using LC3 Antibody (APG8). U251 cells(right) were treated with Chloroquine (50 mu M,16h). The antibody was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
Western blot of anti-LC3 antibody at 8 ug/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).

Western blot

Western blot
Western blot of anti-LC3 antibody HeLa cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.

Requested From: United States
Date Requested: 12/9/2016

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