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Catalog Number Size Price
LS-C165696-400 400 µl $393 
MAP1LC3A / LC3A Antibody - Fluorescent image of U251 cells stained with LC3 (APG8A) (D106) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LC3 (APG8A) (D106) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
MAP1LC3A / LC3A Antibody - Western blot of LC3 (APG8a) (arrow) using rabbit polyclonal Autophagy LC3 Antibody (APG8a) (D106). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LC3 (APG8a) gene (Lane 2) (Origene Technologies).
MAP1LC3A / LC3A Antibody - Fluorescent image of U251 cells stained with LC3 (APG8A) (D106) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LC3 (APG8A) (D106) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
MAP1LC3A / LC3A Antibody - Western blot of LC3 (APG8a) (arrow) using rabbit polyclonal Autophagy LC3 Antibody (APG8a) (D106). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LC3 (APG8a) gene (Lane 2) (Origene Technologies).
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Polyclonal Rabbit anti‑Human MAP1LC3A / LC3A Antibody (aa91‑117, IF, WB) LS‑C165696

Polyclonal Rabbit anti‑Human MAP1LC3A / LC3A Antibody (aa91‑117, IF, WB) LS‑C165696

Antibody:
MAP1LC3A / LC3A Rabbit anti-Human Polyclonal (aa91-117) Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C165696-400
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MAP1LC3A / LC3A Rabbit anti-Human Polyclonal (aa91-117) Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
LC3A antibody LS-C165696 is an unconjugated rabbit polyclonal antibody to human LC3A (MAP1LC3A) (aa91-117). Validated for ICC, IF and WB.
Target
Human MAP1LC3A / LC3A
Synonyms
MAP1LC3A | ATG8E | LC3 | LC3A | MAP1BLC3 | MAP1A/1B light chain 3 A | MAP1A/MAP1B LC3 A | MAP1A/MAP1B light chain 3 A | MAP1ALC3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa91-117
Specificity
This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-117 amino acids from human LC3.
Applications
  • ICC (1:100)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAP1LC3A / LC3A
Q9H492 NM_032514 NP_115903.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/19/2024