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Antibodies > MAP1LC3A / LC3A > LS-C159363
 
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Catalog Number Size Price
LS-C159363-400 400 µl $393 
MAP1LC3A / LC3A Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
MAP1LC3A / LC3A Antibody - 5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in 4% of paraformaldehyde. Treatment with antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.
MAP1LC3A / LC3A Antibody - Fluorescent image of U251 cells stained with cleaved LC3A antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated cleaved LC3A primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
MAP1LC3A / LC3A Antibody - Fluorescent image of U251 cells stained with cleaved LC3A antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with cleaved LC3A primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
MAP1LC3A / LC3A Antibody - Western blot of anti-cleaved-LC3 (APG8a) antibody in mouse brain tissue lysate. Cleaved-LC3 (APG8a) was detected using the purified antibody.
MAP1LC3A / LC3A Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
MAP1LC3A / LC3A Antibody - 5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in 4% of paraformaldehyde. Treatment with antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.
MAP1LC3A / LC3A Antibody - Fluorescent image of U251 cells stained with cleaved LC3A antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated cleaved LC3A primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
MAP1LC3A / LC3A Antibody - Fluorescent image of U251 cells stained with cleaved LC3A antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with cleaved LC3A primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
MAP1LC3A / LC3A Antibody - Western blot of anti-cleaved-LC3 (APG8a) antibody in mouse brain tissue lysate. Cleaved-LC3 (APG8a) was detected using the purified antibody.
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Polyclonal Rabbit anti‑Human MAP1LC3A / LC3A Antibody (aa89‑120, IHC, IF, WB) LS‑C159363

Polyclonal Rabbit anti‑Human MAP1LC3A / LC3A Antibody (aa89‑120, IHC, IF, WB) LS‑C159363

(4.0) Rating
Submit Review (1) Publication(s) LSBio Guarantee
Antibody:
MAP1LC3A / LC3A Rabbit anti-Human Polyclonal (aa89-120) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C159363-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MAP1LC3A / LC3A Rabbit anti-Human Polyclonal (aa89-120) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
LC3A antibody LS-C159363 is an unconjugated rabbit polyclonal antibody to LC3A (MAP1LC3A) (aa89-120) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB. Cited in 1 publication.
(1) Publication(s)
Target
Human MAP1LC3A / LC3A
Synonyms
MAP1LC3A | ATG8E | LC3 | LC3A | MAP1BLC3 | MAP1A/1B light chain 3 A | MAP1A/MAP1B LC3 A | MAP1A/MAP1B light chain 3 A | MAP1ALC3
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa89-120
Specificity
This Cleaved LC3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-120 amino acids from human Cleaved LC3A.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • ICC (1:10 - 1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAP1LC3A / LC3A
Q9H492 NM_032514 NP_115903.1

LSBio Ratings

MAP1LC3A / LC3A Antibody (aa89-120) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-C159363 has an LSBio Rating of
(4.0) Rating
Publications (4)

Publications (1)

Autophagy patterns and prognosis in uveal melanomas. Giatromanolaki AN, Charitoudis GS, Bechrakis NE, Kozobolis VP, Koukourakis MI, Foerster MH, Sivridis EL. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 2011 24:1036-45.
PubMed: 21499230
Submit a Publication for LS-C159363

Customer Reviews (0)

Submit a review for LS-C159363

Featured Products

MAP1LC3A / LC3A Antibody - 10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center.
MAP1LC3A / LC3A Antibody LS‑C165694
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Product
MAP1LC3A / LC3A Antibody (aa74‑106, FITC) LS‑C262516
Species: Human, Mouse
Applications: IHC, Western blot, Flow Cytometry, ELISA
Product
MAP1LC3A / LC3A Antibody (aa74‑106, PE) LS‑C262523
Species: Human, Mouse
Applications: IHC, Western blot, Flow Cytometry, ELISA
MAP1LC3A / LC3A Antibody - Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 Antibody (APG8a) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
MAP1LC3A / LC3A Antibody (aa1‑30) LS‑C165699
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
MAP1LC3A / LC3A Antibody - Anti-LC3 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
IHC‑plus™ MAP1LC3A / LC3A Antibody (aa1‑50) LS‑B7938
Species: Human, Mouse, Rat, Bovine, Opossum, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
MAP1LC3A / LC3A Antibody - IHC of normal human brain using control (rabbit Ig) at 5 ug/ml.
MAP1LC3A / LC3A Antibody (aa1‑50) LS‑C47386
Species: Human, Monkey, Mouse, Rat, Bovine, Opossum, Chicken, Xenopus, Pufferfish
Applications: IHC, IHC - Paraffin, Western blot
See all MAP1LC3A / LC3A Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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