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Anti-Mannose Phosphate Isomerase Antibody (aa150-423) LS-C185557

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $335
Inquire for larger quantities

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206-374-1102
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Most Popular Mannose Phosphate Isomerase Antibodies

Anti-Mannose Phosphate Isomerase Antibody (aa37-65, APC) LS-C263452
Rabbit Polyclonal (IgG) to Human Mannose Phosphate Isomerase
Human
Western blot, ELISA
APC Conjugated
Anti-Mannose Phosphate Isomerase Antibody (aa37-65, FITC) LS-C263453
Rabbit Polyclonal (IgG) to Human Mannose Phosphate Isomerase
Human
Western blot, ELISA
FITC Conjugated
Anti-Mannose Phosphate Isomerase Antibody (aa37-65, PE) LS-C263455
Rabbit Polyclonal (IgG) to Human Mannose Phosphate Isomerase
Human
Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human Mannose Phosphate Isomerase
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human Mannose Phosphate Isomerase
Rabbit
Human (tested or 100% immunogen sequence identity)
Bovine (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

Mannose Phosphate Isomerase antibody was raised against recombinant fragment corresponding to a region within amino acids 150 and 423 of MPI (SwissProt P34949). Percent identity by BLAST analysis: Human (100%); Bovine (92%); Rat (86%); Mouse (84%).
aa150-423
Human MPI / PMI1
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About Mannose Phosphate Isomerase

P34949 NM_002435 NP_002426.1

MPI Antibody, CDG1B Antibody, Mannose phosphate isomerase Antibody, Mannose-6- phosphate isomerase Antibody, Mannose-6-phosphate isomerase Antibody, Phosphohexomutase Antibody, Phosphomannose isomerase 1 Antibody, Phosphomannose isomerase Antibody, PMI Antibody, PMI1 Antibody

Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Gastric CA N87 xenograft using MPI antibody at 1:100 dilution.

Western blot

Western blot
Sample (30g whole cell lysate). A:293T, B: A431 , C: H1299, D: HeLa S3 , E: Hep G2 . F: MOLT4 . G: Raji . 10% SDS PAGE. MPI / PMI1 antibody diluted at 1:1000

Requested From: United States
Date Requested: 12/3/2016

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