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Anti-MAGEA5 Antibody (N-Terminus) LS-C116325

Catalog	Size	Price
LS-C116325-50	50 µg (1 mg/ml)	Unavailable

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Anti-MAGEA5 Antibody (N-Terminus) LS-C116325

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Anti-MAGEA5 Antibody (aa40-120) LS-C384402

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Antibody:	Rabbit Polyclonal to Human MAGEA5
Reactivity:	Human
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human MAGEA5
Host:	Rabbit
Reactivity:	Human (tested or 100% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

MAGEA5 antibody was raised against synthetic peptide from N-Terminus of human MAGEA5 (P43359, NP_066387). Percent identity by BLAST analysis: Human (100%); Marmoset (85%).

Blocking Peptide:

LS-E16882 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116325. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

N-Terminus

Specificity:

Human MAGEA5

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About MAGEA5

Uniprot: P43359 NCBI: NM_021049 NP_066387.1

Synonyms: MAGEA5 Antibody, Cancer/testis antigen 1.5 Antibody, CT1.5 Antibody, MAGE-5a antigen Antibody, MAGE5 Antibody, MAGE-5 antigen Antibody, MAGE-5b antigen Antibody, Melanoma-associated antigen 5 Antibody, Melanoma antigen family A, 5 Antibody

Description: MAGEA5 is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.

(More About MAGEA5)

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Western blot

MAGEA5 antibody LS-C116325 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 6/28/2017

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