Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
MAGEA4 is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAGEA4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunostaining of paraformaldehyde-fixed and paraffin-embedded human breast carcinoma with MAGE-A4 antibody (1:50 dilution), followed by reacting with biotin-conjugated secondary antibody, ABC solution and developing with DAB. Cancerous cells show nuclear staining of MAGE-A4. Magnification: 40X. Data courtesy of Dr. Mi Hou, Karolinska?Institutet and University Hospital, Sweden.
The anti-MAGE-A4 antibody is used in Western blot to detect MAGE-A4 in A375 cell lysate.