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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAGEA2 Antibody (aa23‑72) LS‑C135649
MAGEA2 antibody LS-C135649 is an unconjugated rabbit polyclonal antibody to human MAGEA2. Validated for WB.
Catalog
Size
Price
LS-C135649-50
50 µg
Unavailable

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Product Description

MAGEA2 antibody LS-C135649 is an unconjugated rabbit polyclonal antibody to human MAGEA2. Validated for WB.
About MAGEA2
Reduces p53/TP53 transactivation function through recruitment of HDAC3 to p53/TP53 transcription sites. Also represses p73/TP73 activity. Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination by TRIM28 potentially in presence of Ubl-conjugating enzyme UBE2H. P43356 NM_005361 NP_005352.1

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Specifications

Target
Human MAGEA2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
MAGEA2 antibody was raised against synthetic peptide located between aa23-72 of human MAGEA2 (P43356, NP_005352). Percent identity by BLAST analysis: Human (100%); Gibbon (92%); Gorilla (84%).
Blocking Peptide
LS-E21923 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135649. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa23-72
Specificity
Human MAGEA2
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

MAGEA2 antibody LS-C135649 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAGEA2 antibody LS-C135649 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MAGEA2 antibody LS-C135649 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAGEA2 antibody LS-C135649 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MAGEA2 antibody LS-C135649 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAGEA2 antibody LS-C135649 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MAGEA2 antibody LS-C135649 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAGEA2 antibody LS-C135649 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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