Human MAdCAM-1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

314G8

Biotin

Protein G purified

Unmodified

Recombinant soluble MAdCAM-1-fc fusion protein

Recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1), a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone Reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7. XXX recommend the use of MCA2320EL for this purpose.

• ELISA (1 - 10 µg/ml)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, 0.09% Sodium Azide, 1% BSA

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q13477 NCBI: NM_130762 AAC51354.1