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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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MAD2L1 / MAD2 Antibody (C‑Terminus) LS‑C112300
MAD2 antibody LS-C112300 is an unconjugated rabbit polyclonal antibody to MAD2 (MAD2L1) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C112300-50
50 µg
Unavailable

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Product Description

MAD2 antibody LS-C112300 is an unconjugated rabbit polyclonal antibody to MAD2 (MAD2L1) from human, mouse, rat and other species. Validated for WB.
About MAD2L1 / MAD2
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Q13257 NM_002358 NP_002349.1

MAD2L1 Antibody, HSMAD2 Antibody, MAD2 Antibody, MAD2-like protein 1 Antibody


Specifications

Target
Human MAD2L1 / MAD2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
MAD2L1 / MAD2 antibody was raised against synthetic peptide from C-Terminus of human MAD2L1 (Q13257, NP_002349). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Turkey, Chicken, Platypus, Lizard (100%); Opossum, Xenopus, Frog (90%); Baboon (83%); Salmon, Stickleback, Medaka (81%).
Blocking Peptide
LS-E10518 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C112300. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human MAD2L1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

MAD2L1 antibody LS-C112300 Western blot of MCF7 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAD2L1 antibody LS-C112300 Western blot of MCF7 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MAD2L1 antibody LS-C112300 Western blot of MCF7 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAD2L1 antibody LS-C112300 Western blot of MCF7 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MAD2L1 antibody LS-C112300 Western blot of MCF7 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAD2L1 antibody LS-C112300 Western blot of MCF7 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MAD2L1 antibody LS-C112300 Western blot of MCF7 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAD2L1 antibody LS-C112300 Western blot of MCF7 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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