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Anti-MAD2L1 / MAD2 Antibody (aa3-13) IHC-plus™ LS-B389

Note: This antibody replaces LS-C19079

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MAD2L1 / MAD2 Antibodies

Anti-MAD2L1 / MAD2 Antibody (aa3-13) IHC-plus™ LS-B389
Rabbit Polyclonal to Human MAD2L1 / MAD2
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MAD2L1 / MAD2 Antibody IHC-plus™ LS-B4797
Rabbit Polyclonal to Human MAD2L1 / MAD2
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MAD2L1 / MAD2 Antibody IHC-plus™ LS-B13367
Rabbit Polyclonal (IgG) to Human MAD2L1 / MAD2
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human MAD2L1 / MAD2
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human MAD2L1 / MAD2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:2000 - 1:10000)

Specificity and Use

MAD2L1 / MAD2 antibody was raised against synthetic peptide from human MAD2L1.
aa3-13
Amino acid residues 3-13 of Human MAD2L1 protein.
Immunohistochemistry: LS-B389 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B389 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About MAD2L1 / MAD2

Q13257 NM_002358 NP_002349.1

MAD2L1 Antibody, HSMAD2 Antibody, MAD2 Antibody, MAD2-like protein 1 Antibody

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.

Immunohistochemistry

Immunohistochemistry
Anti-MAD2L1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B389 concentration 5 ug/ml.

Western blot

Western blot
Anti-MAD2L1 Antibody - Western Blot. Western blot of Affinity Purified anti-MAD2L1 antibody shows detection of a predominant band at ~24 kD corresponding to MAD2L1 (arrowhead) present in Jurkat (lane 1) and HeLa (lane 2) whole cell lysates using the 800 nm channel (green). The identity of the higher molecular weight bands is unknown, although they may represent complexes of MAD2L1 with related binding proteins. Specific band reactivity is blocked when the antibody is pre-incubated with immunizing peptide (lanes 4 and 5 respectively) which completely blocks antibody staining. ~35 ug of lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1200. Incubation was 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MXHu ( for 45 min at room temperature. Molecular weight markers were used for size comparison using the 700 nm channel (lane 3). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 12/4/2016

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