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Anti-Macrophage Antibody (clone BM8) LS-C139904


Wt. Vol. Conc. Price
100 µg - 0.1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

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Rat Monoclonal [clone BM8] (IgG2a) to Mouse Macrophage
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Mouse Macrophage
Mouse, Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [BM8]


  • IHC - Paraffin
  • IHC - Frozen
  • Western blot
  • Flow Cytometry

Specificity and Use

Macrophage antibody was raised against bALB/c macrophages obtained from 14-day-old bone marrow cell cultures
The monoclonal antibody BM8 recognises a 125 kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and trombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny.The monoclonal antibody BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.The monoclonal antibody BM8 cross reacts with human heart macrophages.
WB: Mouse bone-marrow derived macrophages; non-reduced; ~125 kDa; reduction with 2-mercaptoethanol destroys BM8 antigenF: tissue embedded in OCT Tissue Tec; fixed with acetone for 10 min at RT; incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase; spleen as positive controlFor flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The recommended use of this reagent is 10 ul per 250000 cells in a 100 ul total staining volume.


PBS, 0.1% BSA, 0.02% sodium azide.
Store at 4°C
For research use only.

About Macrophage

Macrophages (Greek: big eaters, from makros "large" + phagein "eat"; abbr. MF), are a type of white blood cell that engulfs and digests cellular debris, foreign substances, microbes, cancer cells, and anything else that does not have the types of proteins specific to the surface of healthy body cells on its surface in a process called phagocytosis. Macrophages were first discovered by Élie Metchnikoff, a Russian bacteriologist, in 1884.


F4/80 expression on macrophages in mouse liver. Staining of frozen tissue section with antibody BM8.

Requested From: United States
Date Requested: 10/23/2016

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