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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LYZ / Lysozyme Antibody IHC‑plus™ LS‑B7933
Note: This antibody replaces LS-C147788
Lysozyme antibody LS-B7933 is an unconjugated rabbit polyclonal antibody to human Lysozyme (LYZ). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B7933-10
10 mg (10 mg/ml)
Unavailable

Popular LYZ / Lysozyme Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Flow Cytometry
Antibody
Species: Chicken
Applications: ELISA
Anti-Lysozyme antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8648 dilution 1:100.
Species: Human, Baboon, Monkey, Cat, Dog, Pig
Applications: IHC, IHC - Paraffin
Western Blot of Rabbit anti-Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: ~1 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4 degrees C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: MB-070 overnight at 4 degrees C. Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Chicken
Applications: IHC, Western blot, Immunoprecipitation, ELISA

Product Description

Lysozyme antibody LS-B7933 is an unconjugated rabbit polyclonal antibody to human Lysozyme (LYZ). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About LYZ / Lysozyme
LYZ / Lysozyme is human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. P61626 NM_000239 NP_000230.1

LYZ Antibody, 1,4-beta-N-acetylmuramidase C Antibody, LZM Antibody, Lysozyme Antibody, Lysozyme (renal amyloidosis) Antibody, Renal amyloidosis Antibody, Lysozyme C Antibody


Specifications

Target
Human LYZ / Lysozyme
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:400)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
LYZ / Lysozyme antibody was raised against human lysozyme
Specificity
Human LYZ / Lysozyme. The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. Cross-reactivity The antiserum does not cross-react with any other component of human plasma. Inter-species cross- reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Usage
The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e. g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays are between 1:500 and 1:5000.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile distilled water 1 ml
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-Lysozyme antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7933 dilution 1:400.
Anti-Lysozyme antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7933 dilution 1:400.

Immunohistochemistry

Anti-Lysozyme antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7933 dilution 1:400.
Anti-Lysozyme antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7933 dilution 1:400.

Immunohistochemistry

Anti-Lysozyme antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7933 dilution 1:400.
Anti-Lysozyme antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7933 dilution 1:400.

Immunohistochemistry

Anti-Lysozyme antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7933 dilution 1:400.
Anti-Lysozyme antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7933 dilution 1:400.


Requested From: 
Date Requested: 6/22/2018

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