Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 31F5] (IgG1) to Yeast Lys20p + Lys21p
Immunofluorescence, Western blot
Yeast Lys20p + Lys21p
Yeast (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [31F5]
Tissue culture supernatant
Immunofluorescence (1:1000 - 1:10000)
Western blot (1:10000 - 1:50000)
Specificity and Use
For immunoblots of yeast protein samples, use Lys20p + Lys21p antibody diluted 1:10000-1:50000 followed by chemiluminescent detection (ECL). For non-ECL western detection methods, try the antibody diluted 1:1000-1:5000. For immunofluorescence on yeast cells, use the antibody diluted 1:1000-1:10000 (we routinely dilute the antibody at 1:5000).
Tissue culture supernatant, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
Western blots of whole yeast protein extracts with a collection of our antibodies. The blot for Lys20p + Lys21p antibody is in the indicated lane, and the number indicates the SDS-PAGE molecular weight in kiloDaltons.