Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone D7] (IgG2a) to Mouse Ly6A/E / Sca-1
Western blot, Immunoprecipitation, Flow Cytometry (applications tested for the base form of this product only)
Mouse Ly6A/E / Sca-1
Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [D7]
Flow Cytometry (1:5 - 1:10)
(applications tested for the base form of this product only)
Specificity and Use
Ly6A/E / Sca-1 antibody was raised against iL-2-dependent mouse T cell line CTL-L.
Recognizes members of the lymphocyte activation protein-6 (Ly6) multigene family. Specifically recognizes Ly6A.2 and Ly6E.1. Ly6A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kD mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells. Reported to induce T cell activation.
The applications listed have been tested for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
PBS, pH 7.4, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.