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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Ly6A/E / Sca‑1 Antibody (clone D7, FITC) LS‑C58325
Antibody
Sca-1 antibody LS-C58325 is an FITC-conjugated rat monoclonal antibody to mouse Sca-1 (Ly6A / E). Validated for Flow, IP and WB.
Catalog
Size
Price
LS-C58325-100
100 µg
Unavailable

Popular Ly6A/E / Sca-1 Products

Antibody
Species: Mouse
Applications: IHC, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Staining of ConA-activated splenocytes with 0.25 ug of PE anti-CD3 (LS-C107143) and 0.25 ug of FITC D7. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Flow Cytometry
Antibody
Species: Mouse
Applications: IHC, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Antibody
Species: Mouse
Applications: IHC, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Antibody
Species: Mouse
Applications: IHC, Western blot, Immunoprecipitation, Flow Cytometry

Product Description

Sca-1 antibody LS-C58325 is an FITC-conjugated rat monoclonal antibody to mouse Sca-1 (Ly6A / E). Validated for Flow, IP and WB.

Specifications

Target
Mouse Ly6A/E / Sca-1
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [D7]
Conjugations
Fluorescein. Also available Unconjugated or conjugated with Biotin, Cy5, RPE.
Purification
Purified
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:5 - 1:10)
  • (applications tested for the base form of this product only)
Immunogen
Ly6A/E / Sca-1 antibody was raised against iL-2-dependent mouse T cell line CTL-L.
Specificity
Recognizes members of the lymphocyte activation protein-6 (Ly6) multigene family. Specifically recognizes Ly6A.2 and Ly6E.1. Ly6A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kD mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells. Reported to induce T cell activation.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Presentation
PBS, pH 7.4, 0.09% sodium azide.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/18/2018

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