Mouse Ly6A/E / Sca-1
(tested or 100% immunogen sequence identity)
Also available Unconjugated
or conjugated with Biotin
, Cy5, RPE
- Western blot
- Flow Cytometry (1:5 - 1:10)
- (applications tested for the base form of this product only)
Ly6A/E / Sca-1 antibody was raised against iL-2-dependent mouse T cell line CTL-L.
Recognizes members of the lymphocyte activation protein-6 (Ly6) multigene family. Specifically recognizes Ly6A.2 and Ly6E.1. Ly6A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kD mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells. Reported to induce T cell activation.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
PBS, pH 7.4, 0.09% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.