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Anti-Ly6A/E / Sca-1 Antibody (clone D7, FITC) LS-C58325

Catalog Size Price
LS-C58325-100 100 µg Unavailable

Related Products

23 Ly6A/E / Sca-1 Antibodies

Most Popular Ly6A/E / Sca-1 Antibodies

Anti-Ly6A/E / Sca-1 Antibody (clone D7) LS-C34585
Rat Monoclonal [clone D7] (IgG2a) to Mouse Ly6A/E / Sca-1
Mouse
IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Anti-Ly6A/E / Sca-1 Antibody (clone D7, Biotin) LS-C62867
Rat Monoclonal [clone D7] (IgG2a) to Mouse Ly6A/E / Sca-1
Mouse
IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Biotin Conjugated
Anti-Ly6A/E / Sca-1 Antibody (clone D7, FITC) LS-C105781
Rat Monoclonal [clone D7] (IgG2a,k) to Mouse Ly6A/E / Sca-1
Mouse
Flow Cytometry
FITC Conjugated
Flow Cytometry Image
Anti-Ly6A/E / Sca-1 Antibody (clone D7) LS-C350335
Rat Monoclonal [clone D7] (IgG2a) to Mouse Ly6A/E / Sca-1
Mouse
IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Anti-Ly6A/E / Sca-1 Antibody (clone D7, Azide-free, Low Endotoxin) LS-C350342
Rat Monoclonal [clone D7] (IgG2a) to Mouse Ly6A/E / Sca-1
Mouse
IHC, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated, Azide-free, Low Endotoxin

100% Guaranteed
Rat Monoclonal [clone D7] (IgG2a) to Mouse Ly6A/E / Sca-1
Mouse
Western blot, Immunoprecipitation, Flow Cytometry (applications tested for the base form of this product only)
FITC Conjugated

Details

Mouse Ly6A/E / Sca-1
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [D7]
Fluorescein. Also available Unconjugated or conjugated with Biotin, Cy5, RPE.
Purified
Unmodified

Applications

  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:5 - 1:10)
  • (applications tested for the base form of this product only)

Specificity and Use

Ly6A/E / Sca-1 antibody was raised against iL-2-dependent mouse T cell line CTL-L.
Recognizes members of the lymphocyte activation protein-6 (Ly6) multigene family. Specifically recognizes Ly6A.2 and Ly6E.1. Ly6A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kD mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells. Reported to induce T cell activation.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.

Packaging

PBS, pH 7.4, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About Ly6A/E / Sca-1


Requested From: 
Date Requested: 8/22/2017

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