Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone 4D11] (IgG2a,k) to Mouse LY-49G2
Immunoprecipitation, Flow Cytometry
Mouse (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [4D11]
Specificity and Use
LY-49G2 antibody was raised against mouse LY-49G2
This 4D11 (4D11) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1.0 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Staining of C57Bl/6 splenocytes with 0.5 ug of Purified Rat IgG2a isotype control (open histogram) or 0.5 ug of Purified anti-mouse Ly-49G2 (4D11) (colored histogram) followed by Biotin Anti-Rat IgG and SAv-PE. Total viable cells were used for analysis.
Requested From: United States
Date Requested: 2/28/2017