Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Chicken (at least 90% immunogen sequence identity)
IHC - Paraffin (5 µg/ml)
Western blot (0.5 - 2 µg/ml)
Specificity and Use
LXR Alpha+Beta antibody was raised against a synthetic peptide made to an internal portion of the human LXR protein sequence (between residues 50-150). Percent identity by BLAST analysis: Rat, Mouse (92%); Chicken (90%).
LXR alpha and beta isoforms.
Immunohistochemistry: LS-B262 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B262 was determined to be 5 ug/ml.
Tris-glycine buffer, 150 mM sodium chloride, 0.05% sodium azide.
Anti-LXR Alpha/Beta antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B262 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From: United States
Date Requested: 1/22/2017