Target
Human LSP1
Synonyms
LSP1 | 47 kDa actin-binding protein | 47 kDa actin binding protein | 52 kDa phosphoprotein | Leukocyte-specific protein 1 | Lymphocyte-specific protein 1 | Pp52 | WP34
Reactivity
Human, Bovine, Dog
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-ASTKSRWETGEVQAQ, from the internal region of the protein sequence according to NP_002330.1NP_001013271.1.
Specificity
Human LSP1. This antibody is expected to recognize both reported isoforms (NP_002330.1; NP_001013271.1). Reported variants represent identical protein: NP_001013273.1, NP_001013271.1, NP_001013272.1
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 55kDa band observed in Human Lymph Node, Tonsil and in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 37.2kDa according to NP_002330.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Liu et al, J Exp Med. 2005 Feb 7;201(3):409-18. PMID: 15684321). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee