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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LSM1 Antibody (clone 5E6) LS‑C173081
LSM1 antibody LS-C173081 is an unconjugated mouse monoclonal antibody to human LSM1. Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C173081-100
100 µl (0.57 mg/ml)
Unavailable

Popular LSM1 Products

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with LSM1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-LSM1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5714 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bat, Dog, Guinea pig, Hamster, Rabbit, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Anti-LSM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LSM1 mouse monoclonal antibody.
Species: Human, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

LSM1 antibody LS-C173081 is an unconjugated mouse monoclonal antibody to human LSM1. Validated for Flow, IF and WB.
About LSM1
LSM1 is a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Increased expression of this gene may play a role in cellular transformation and the progression of several malignancies including lung cancer, mesothelioma and breast cancer. NM_014462 NP_055277.1

LSM1 Antibody, Cancer-associated Sm-like Antibody, Small nuclear ribonuclear CaSm Antibody, CASM Antibody, YJL124C Antibody


Specifications

Target
Human LSM1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [5E6]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Immunogen
LSM1 antibody was raised against human recombinant protein fragment corresponding to amino acids 1-133 of human LSM1 (NP_055277) produced in E. coli.
Specificity
Human LSM1
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Anti-LSM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1.
Anti-LSM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-LSM1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-LSM1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LSM1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LSM1 antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-LSM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1.
Anti-LSM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-LSM1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-LSM1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LSM1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LSM1 antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-LSM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1.
Anti-LSM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-LSM1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-LSM1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LSM1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LSM1 antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-LSM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1.
Anti-LSM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-LSM1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-LSM1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LSM1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LSM1 antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/19/2018

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