LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
LRRC50 Antibody (aa151‑200) LS‑C82744
LRRC50 antibody LS-C82744 is an unconjugated rabbit polyclonal antibody to LRRC50 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C82744-50
50 µg
Unavailable

Popular LRRC50 Products

Anti-LRRC50 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LRRC50.
Species: Human
Applications: Immunofluorescence, Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Species: Human, Monkey, Mouse, Rat, Dog
Applications: Western blot, Flow Cytometry
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
Species: Human
Applications: Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
Species: Human
Applications: Western blot
Western blot of extracts (35ug) from 9 different cell lines by using anti-LRRC50 monoclonal antibody.
Species: Human, Monkey, Mouse, Rat
Applications: Western blot, Flow Cytometry

Product Description

LRRC50 antibody LS-C82744 is an unconjugated rabbit polyclonal antibody to LRRC50 from human, mouse, rat and other species. Validated for WB.
About LRRC50
Cilium-specific protein required for the stability of the ciliary architecture. Plays a role in cytoplasmic preassembly of dynein arms. Involved in regulation of microtubule-based cilia and actin-based brush border microvilli. Q8NEP3 Q8NEP3

DNAAF1 Antibody, CILD13 Antibody, LRRC50 Antibody, ODA7 Antibody


Specifications

Target
Human LRRC50
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bat, Bovine, Guinea pig, Pig (tested or 100% immunogen sequence identity)
Predicted
Monkey, Dog, Horse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
LRRC50 antibody was raised against synthetic peptide located between aa151-200 of human DNAAF1 (Q8NEP3, NP_848547). Percent identity by BLAST analysis: Human, Gorilla, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Bat, Pig, Guinea pig (100%); Monkey, Dog, Horse, Zebra finch (92%); Gibbon, Turkey, Chicken, Lizard (85%).
Blocking Peptide
LS-E19269 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C82744. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa151-200
Specificity
Human LRRC50
Usage
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number