Human LPIN1 / Lipin 1

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

E. coli-derived rhLipin 1 (aa 448-574).

aa448-574

Selected for its ability to recognize human Lipin 1 in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 2% cross-reactivity with rhLipin2 and rhLipin3.

• ICC
• Western blot
• ELISA

Immunocytochemistry: This antibody has been used at a concentration of 10 ug/ml to detect human Lipin 1 on human MSC-derived adipocytes. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human Lipin 1. The detection limit for rhLipin 1 is approximately 2 ng/lane under non-reducing and reducing conditions. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Lipin 1. The detection limit for rhLipin 1 is approximately 0.1 ng/well.

Lyophilized

Reconstitute in deionized water

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q14693 NCBI: D80010 BAA11505.1