(tested or 100% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- IHC - Frozen (1:200 - 1:1000)
- Western blot (1:200 - 1:1000)
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including antigen retrieval solutions, blocking agents
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LMO4 antibody was raised against synthetic peptide corresponding to aa115-127 of human LMO4.
Recognizes LMO4. Species cross-reactivity: Human and rodent.
Immunohistochemistry: LS-B2619 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2619 was determined to be 5 ug/ml.
PBS, pH 7.2. No preservative added.
4°C or -20°C, Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.