Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
Western blot (1:5000)
Specificity and Use
LMNA / Lamin A+C antibody was raised against recombinant full length purified Lamin A from E. coli.
This antibody is known to react with Lamin A and Lamin C from human, cow, pig, mouse, rat and other mammals. Since Lamin A/C is highly conserved, it is likely that the antibody is effective on other species also.
The antibody solution can be used at dilutions of at least 1:5000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:5000.
Antiserum, 10 mM sodium azide
+4°C or -20°C, Avoid repeated freezing and thawing.
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B.
HeLa cells staining with LMNA / Lamin A/C antibody (red) counterstained with monoclonal antibody to HSP27, 6H11 (green) and DNA (blue). The LMNA / Lamin A/C antibody antibody reveals strong nuclear lamina staining, while the MCA-6H11 antibody reveals strong cytoplasmic staining. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.
Stripe blot of crude HeLa cell extract stained with LMNA / Lamin A/C antibody. Note two strong and clean bands at 74kDa and 65kDa, corresponding to Lamin A and C.
Requested From: United States
Date Requested: 1/19/2017