Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Protein G purified
IHC - Frozen
Western blot (1:25 - 1:250)
Flow Cytometry (1:25 - 1:50)
Specificity and Use
LMNA / Lamin A+C antibody was raised against last eight C-terminal amino acids of Lamin C, including an N-terminal lysine as a linker (KHHVSGSRR) coupled to KLH.
Specific for lamin C. Species cross-reactivity: human.
Suitable for use in Immunohistochemistry (frozen sections), Flow Cytometry, and Western Blot. Flow Cytometry: 1:25-1:50. Immunohistochemistry: 1:25-1:50, using avidin-biotin HRP complex for detection (ABC). Western Blot: 1:25-1:250. Optimum dilutions to be determined by researcher.
0.09% sodium azide, glycerol/PBS to 40%. No stabilizing proteins added.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B.