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Anti-LMNA / Lamin A+C Antibody (aa249-266) IHC-plus™ LS-B119

Note: This antibody replaces LS-C733, LS-C71501

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular LMNA / Lamin A+C Antibodies

Anti-LMNA / Lamin A+C Antibody (aa572-646, clone JOL4) LS-C82328
Mouse Monoclonal [clone JOL4] (IgG) to Human LMNA / Lamin A+C
Human
IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-LMNA / Lamin A+C Antibody (aa464-572, clone JOL2) IHC-plus™ LS-B2973
Mouse Monoclonal [clone JOL2] (IgG1) to Human LMNA / Lamin A+C
Human
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human LMNA / Lamin A+C
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated

Details

Human LMNA / Lamin A+C
Rabbit
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Bovine (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC - Paraffin (1:100)
  • Western blot (1:500 - 1:1000)

Specificity and Use

LMNA / Lamin A+C antibody was raised against a synthetic peptide corresponding to amino acids 249-265 C-RAQHEDQVEQYKKELEK of Human Lamin A/C. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Horse, Rabbit, Pig (100%); Bovine, Opossum (94%); Bat, Platypus (88%).
aa249-266
Because of immunogen's 100% homology with Isoforms 1, 4, 5, 6, ADelta10 and C of Prelamin-A/C, this is expected to detect multiple bands in WB.
Immunohistochemistry: LS-B119 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B119 was determined to be 1:100.

Packaging

PBS containing 0.05% BSA and 0.05% sodium azide
Long term: -70°C; Short term: +4°C
For research use only.

About LMNA / Lamin A+C

P02545 NM_005572 NP_005563.1

LMNA Antibody, 70 kDa lamin Antibody, CDCD1 Antibody, CMD1A Antibody, EMD2 Antibody, FPLD Antibody, IDC Antibody, FPL Antibody, FPLD2 Antibody, Lamin A/C Antibody, Lamin Antibody, LFP Antibody, LMNC Antibody, LMN1 Antibody, LMNL1 Antibody, HGPS Antibody, Prelamin-A/C Antibody, PRO1 Antibody, Lamin A Antibody, Lamin A/C-like 1 Antibody, LGMD1B Antibody, CDDC Antibody, CMT2B1 Antibody, LDP1 Antibody

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B.

Immunohistochemistry

Immunohistochemistry
Anti-Lamin A/C antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B119 dilution 1:100.

Western blot

Western blot
Western blot of human Lamin A/C in 15 ugs of HeLa cell lysate using antibody at 1:1000 dilution.

Requested From: United States
Date Requested: 12/8/2016

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