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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LMCD1 Antibody (clone 2F12) LS‑C172904 Best Seller
LMCD1 antibody LS-C172904 is an unconjugated mouse monoclonal antibody to human LMCD1. Validated for Flow and WB.
Catalog
Size
Price
LS-C172904-100
100 µl (0.63 mg/ml)
Unavailable

Popular LMCD1 Products

Antibody
Species: Human
Applications: Immunofluorescence, Western blot
Anti-LMCD1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMCD1.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Human lymphoma tissue using anti-LMCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Western blot of lysates from human skeletal muscle, mouse lung, rat lung tissue lysate (from left to right), using LMCD1 antibody diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
Species: Human, Mouse, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

LMCD1 antibody LS-C172904 is an unconjugated mouse monoclonal antibody to human LMCD1. Validated for Flow and WB.
About LMCD1
Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway. Q9NZU5 NM_014583 NP_055398.1

LMCD1 Antibody, Dyxin Antibody


Specifications

Target
Human LMCD1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2F12]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Western blot (1:500)
  • Flow Cytometry (1:100)
Immunogen
LMCD1 antibody was raised against full length human recombinant protein of human LMCD1 (NP_055398) produced in HEK293T cell.
Specificity
Human LMCD1
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMCD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMCD1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMCD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMCD1.

Flow Cytometry

Flow cytometry of HeLa cells, using anti-LMCD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-LMCD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMCD1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMCD1 antibody, and then analyzed by flow cytometry.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMCD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMCD1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMCD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMCD1.

Flow Cytometry

Flow cytometry of HeLa cells, using anti-LMCD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-LMCD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMCD1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMCD1 antibody, and then analyzed by flow cytometry.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMCD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMCD1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMCD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMCD1.

Flow Cytometry

Flow cytometry of HeLa cells, using anti-LMCD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-LMCD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMCD1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMCD1 antibody, and then analyzed by flow cytometry.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMCD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMCD1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMCD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMCD1.

Flow Cytometry

Flow cytometry of HeLa cells, using anti-LMCD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-LMCD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMCD1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMCD1 antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/19/2018

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