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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LIPG / Endothelial Lipase Antibody (clone 1D9) LS‑C115064
Endothelial Lipase antibody LS-C115064 is an unconjugated mouse monoclonal antibody to human Endothelial Lipase (LIPG). Validated for Flow, IF, IHC, IP and WB.
Catalog
Size
Price
LS-C115064-100
100 µl
Unavailable

Popular LIPG / Endothelial Lipase Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-LIPG antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1599 concentration 5 ug/ml.
Species: Human, Mouse, Rat, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse, Rat, Pig, Sheep
Applications: IHC, Western blot
Antibody (0.3 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Species: Human, Monkey
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of recombinant LIPG / Endothelial Lipase.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot

Product Description

Endothelial Lipase antibody LS-C115064 is an unconjugated mouse monoclonal antibody to human Endothelial Lipase (LIPG). Validated for Flow, IF, IHC, IP and WB.
About LIPG / Endothelial Lipase
LIPG (endothelial lipase) has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. Q9Y5X9 NM_006033 NP_006024.1

LIPG Antibody, EDL Antibody, EL Antibody, Lipoprotein lipase H Antibody, Lipase, endothelial Antibody, PRO719 Antibody, Endothelial lipase Antibody


Specifications

Target
Human LIPG / Endothelial Lipase
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [1D9]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Immunoprecipitation
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human recombinant protein of human LIPG (NP_006024) produced in HEK293T cell.
Specificity
Human LIPG / Endothelial Lipase
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-LIPG mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIPG.
Anti-LIPG mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIPG.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody, and then analyzed by flow cytometry.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY LIPG (Red) or empty vector control plasmid (Blue) were immunostained with anti-LIPG mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY LIPG (Red) or empty vector control plasmid (Blue) were immunostained with anti-LIPG mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-LIPG mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIPG.
Anti-LIPG mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIPG.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody, and then analyzed by flow cytometry.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY LIPG (Red) or empty vector control plasmid (Blue) were immunostained with anti-LIPG mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY LIPG (Red) or empty vector control plasmid (Blue) were immunostained with anti-LIPG mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-LIPG mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIPG.
Anti-LIPG mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIPG.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody, and then analyzed by flow cytometry.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY LIPG (Red) or empty vector control plasmid (Blue) were immunostained with anti-LIPG mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY LIPG (Red) or empty vector control plasmid (Blue) were immunostained with anti-LIPG mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-LIPG mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-LIPG mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIPG.
Anti-LIPG mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIPG.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody, and then analyzed by flow cytometry.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY LIPG (Red) or empty vector control plasmid (Blue) were immunostained with anti-LIPG mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY LIPG (Red) or empty vector control plasmid (Blue) were immunostained with anti-LIPG mouse monoclonal, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/23/2018

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