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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LIPA / Lysosomal Acid Lipase Antibody (aa42‑91) LS‑C135924
Lysosomal Acid Lipase antibody LS-C135924 is an unconjugated rabbit polyclonal antibody to Lysosomal Acid Lipase (LIPA) from human, rat, horse and other species. Validated for WB.
Catalog
Size
Price
LS-C135924-50
50 µg
$365

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Applications: IHC, Western blot, ELISA

Product Description

Lysosomal Acid Lipase antibody LS-C135924 is an unconjugated rabbit polyclonal antibody to Lysosomal Acid Lipase (LIPA) from human, rat, horse and other species. Validated for WB.
About LIPA / Lysosomal Acid Lipase
Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation. P38571 NM_000235 NP_000226.2

LIPA Antibody, Cholesterol ester hydrolase Antibody, Cholesteryl esterase Antibody, LAL Antibody, Lysosomal acid lipase Antibody, Lipase A Antibody, Sterol esterase Antibody, CESD Antibody


Specifications

Target
Human LIPA / Lysosomal Acid Lipase
Host
Rabbit
Reactivity
Human, Monkey, Rat, Horse, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa42-91 of human LIPA (P38571, NP_000226). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Pig (100%); Galago, Sheep, Hamster, Panda, Bovine, Bat, Rabbit (92%); Rat, Elephant, Horse (85%).
Epitope
aa42-91
Specificity
Human LIPA
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Western blot

LIPA antibody LS-C135924 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
LIPA antibody LS-C135924 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

LIPA antibody LS-C135924 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
LIPA antibody LS-C135924 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

LIPA antibody LS-C135924 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
LIPA antibody LS-C135924 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

LIPA antibody LS-C135924 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
LIPA antibody LS-C135924 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/26/2018

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