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Anti-LINGO2 Antibody LS-C149991

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular LINGO2 Antibodies

Anti-LINGO2 Antibody (aa28-542) LS-C37184
Mouse Monoclonal (IgG2a) to Human LINGO2
Human
ICC, Western blot, Flow Cytometry, ELISA
Unconjugated
Anti-LINGO2 Antibody (C-Terminus) IHC-plus™ LS-B14041
Rabbit Polyclonal to Mouse LINGO2
Mouse, Human, Rat, Bovine, Guinea pig, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human LINGO2
Human
ICC, Western blot, ELISA
Unconjugated

Details

Human LINGO2
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

LINGO2 antibody was raised against purified, NS0-derived, recombinant human LRR and Ig domain containing Nogo receptor-interacting protein 2 (rhLINGO-2; aa 28 - 542; Accession # NP_689783).
Human LINGO-2Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 0.2 mg/mL.
In direct ELISAs and Western blots, this antibody shows less than 5% cross-reactivity with rhLINGO-1. Applications. Western blot-This antibody can be used at 0.1-0.2 µg/ml with the appropriate secondary reagents to detect human LINGO-2. The detection limit for rhLINGO-2 is approximately 25 ng/lane under non-reducing and reducing conditions. Immunocytochemistry-This antibody has been used at a concentration of 10 µg/ml to detect human LINGO-2 in A549 cells. Cells were fixed with PBS containing. 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4° C followed by NorthernLIghts-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA-This antibody can be used at 0.5-1.0 µg/ml with the appropriate secondary reagents to detect human LINGO-2. The detection limit for rhLINGO-2 is approximately 0.5 ng/well.

Packaging

Lyophilized from 0.2 µm filtered 100 mM NaHCO3, pH 8.3, 500 mM NaCl, 1% sucrose.
Store at -20°C.
For research use only.

About LINGO2

Q7L985 NM_152570 NP_689783.1

LINGO2 Antibody, LERN3 Antibody, LRRN6C Antibody


Requested From: United States
Date Requested: 12/2/2016

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