Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
ICC (10 µg/ml)
Specificity and Use
LINGO2 antibody was raised against purified, NS0-derived, recombinant human LRR and Ig domain containing Nogo receptor-interacting protein 2 (rhLINGO-2; aa 28 - 542; Accession # NP_689783).
Human LINGO-2Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 0.2 mg/mL.
In direct ELISAs and Western blots, this antibody shows less than 5% cross-reactivity with rhLINGO-1. Applications. Western blot-This antibody can be used at 0.1-0.2 µg/ml with the appropriate secondary reagents to detect human LINGO-2. The detection limit for rhLINGO-2 is approximately 25 ng/lane under non-reducing and reducing conditions. Immunocytochemistry-This antibody has been used at a concentration of 10 µg/ml to detect human LINGO-2 in A549 cells. Cells were fixed with PBS containing. 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4° C followed by NorthernLIghts-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA-This antibody can be used at 0.5-1.0 µg/ml with the appropriate secondary reagents to detect human LINGO-2. The detection limit for rhLINGO-2 is approximately 0.5 ng/well.
Lyophilized from 0.2 µm filtered 100 mM NaHCO3, pH 8.3, 500 mM NaCl, 1% sucrose.