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Anti-LINE-1 Antibody LS-C154072

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $405
Inquire for larger quantities

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Most Popular LINE-1 Antibodies

Anti-LINE-1 Antibody LS-C154072
Chicken Polyclonal (IgY) to Human LINE-1
Human
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Chicken Polyclonal (IgY) to Human LINE-1
Human
Western blot, ELISA
Unconjugated

Details

Human LINE-1
Chicken
Human (tested or 100% immunogen sequence identity)
IgY Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot (1:2000 - 1:10000)
  • ELISA

Specificity and Use

LINE-1 antibody was raised against this IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences.
This IgY fraction antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 149 kD in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About LINE-1

The L1 (LINE-1), or Long INterspersed Element , retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains.

Western blot

Western blot
Anti-L1/ORF2 Antibody - Western Blot. Western blot of IgY fraction of anti-L1/ORF2 antibody shows detection of induced bacterially expressed human ORF2 (left lane). No specific band staining is seen in the uninduced lane (right lane). The lower molecular weight bands represent non-specific staining. The band at ~70 kD corresponds to a human L1/ORF2 EN domain fusion protein (arrowhead). Personal communication, D. Symer, NCI, Bethesda, MD.
 

Requested From: United States
Date Requested: 12/2/2016

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