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Anti-LIN28A / LIN28 Antibody (N-Terminus) IHC-plus™ LS-B3510

Note: This antibody replaces LS-C101501

Ordering

Wt. Vol. Conc. Price
50 µg - - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular LIN28A / LIN28 Antibodies

Anti-LIN28A / LIN28 Antibody (aa100-150) LS-C148680
Rabbit Polyclonal to Human LIN28A / LIN28
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-LIN28A / LIN28 Antibody (aa108-138) IHC-plus™ LS-B11566
Rabbit Polyclonal to Human LIN28A / LIN28
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human LIN28A / LIN28
Human, Bovine, Dog, Pig, Sheep
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human LIN28A / LIN28
Rabbit
Human, Bovine, Dog, Pig, Sheep (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

LIN28A / LIN28 antibody was raised against synthetic peptide from N-Terminus of human LIN28A (Q9H9Z2, NP_078950). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Panda, Dog, Cat, Bovine, Pig (100%); Rabbit (92%); Monkey, Mouse, Rat, Elephant (90%); Sheep, Guinea pig (85%).
LS-E12225 - Lyophilized - 100 µg - $145.00
N-Terminus
Human LIN28A / LIN28
Immunohistochemistry: LS-B3510 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3510 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About LIN28A / LIN28

Q9H9Z2 NM_024674 NP_078950.1

LIN28A Antibody, CSDD1 Antibody, Lin-28 homolog (C. elegans) Antibody, LIN-28 Antibody, Lin-28A Antibody, LIN28 Antibody, Protein lin-28 homolog A Antibody, Lin-28 homolog A (C. elegans) Antibody, ZCCHC1 Antibody, RNA-binding protein LIN-28 Antibody

Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA.

Immunohistochemistry

Immunohistochemistry
Anti-LIN28A / LIN28 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3510 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-LIN28A / LIN28 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3510 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-LIN28A / LIN28 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3510 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
LIN28A / LIN28 antibody LS-B3510 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/4/2016

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