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Anti-LIMS1 / PINCH Antibody (clone 5G7) LS-C169391

Note: This antibody replaces LS-C171624

Ordering

Wt. Vol. Conc. Price
- 100 µl - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular LIMS1 / PINCH Antibodies

Anti-LIMS1 / PINCH Antibody (clone 5G7) LS-C169391
Mouse Monoclonal [clone 5G7] (IgG1) to Human LIMS1 / PINCH
Human
ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunofluorescence Image
Anti-LIMS1 / PINCH Antibody (aa68-303, FITC) LS-C302116
Rabbit Polyclonal (IgG) to Human LIMS1 / PINCH
Human
Western blot, ELISA
FITC Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 5G7] (IgG1) to Human LIMS1 / PINCH
Human
ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human LIMS1 / PINCH
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [5G7]
Unconjugated
Ascites
Unmodified

Applications

  • ICC (1:200 - 1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)

Specificity and Use

LIMS1 / PINCH antibody was raised against purified recombinant fragment of human PINCH expressed in E. coli.
Human PINCH

Packaging

Ascites, 0.03% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About LIMS1 / PINCH

P48059 NM_004987 NP_004978.2

LIMS1 Antibody, PINCH Antibody, PINCH-1 Antibody, PINCH1 Antibody

LIMS1 / PINCH is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways.

Immunofluorescence

Immunofluorescence
Immunofluorescence of HepG2 cells using PINCH mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot
Western blot using PINCH mouse monoclonal antibody against A549 (1), Jurkat (2), and HeLa (3) cell lysate.

Western blot

Western blot
Western blot using PINCH monoclonal antibody against human PINCH (AA: 87-249) recombinant protein. (Expected MW is 44.2 kDa)

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Requested From: United States
Date Requested: 12/10/2016

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