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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LIMPII / SCARB2 Antibody (aa96‑110) IHC‑plus™ LS‑B8307
Note: This antibody replaces LS-C112833
SCARB2 antibody LS-B8307 is an unconjugated goat polyclonal antibody to SCARB2 (LIMPII) from human and rabbit. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B8307-50
50 µg (0.5 mg/ml)
Unavailable

Popular LIMPII / SCARB2 Products

Anti-SCARB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B305 concentration 1:500.
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Anti-SCARB2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3225 concentration 5 ug/ml.
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Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA619859LA01HU at dilution of 1:100
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Product Description

SCARB2 antibody LS-B8307 is an unconjugated goat polyclonal antibody to SCARB2 (LIMPII) from human and rabbit. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About LIMPII / SCARB2
LIMPII / SCARB2 is a type III glycoprotein that is located primarily in limiting membranes of lysosomes and endosomes. Earlier studies in mice and rat suggested that this protein may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. The protein deficiency in mice was reported to impair cell membrane transport processes and cause pelvic junction obstruction, deafness, and peripheral neuropathy. Q14108 NM_005506 NP_005497.1

SCARB2 Antibody, AMRF Antibody, CD36 antigen-like 2 Antibody, LGP85 Antibody, Lysosome membrane protein 2 Antibody, LIMPII Antibody, HLGP85 Antibody, LIMP-2 Antibody, SR-BII Antibody, CD36 antigen Antibody, CD36L2 Antibody, EPM4 Antibody, LIMP II Antibody, Lysosome membrane protein II Antibody


Specifications

Target
Human LIMPII / SCARB2
Host
Goat
Reactivity
Human, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Hamster, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
LIMPII / SCARB2 antibody was raised against synthetic peptide C-NKANIQFGDNGTTIS from an internal region of human SCARB2 / SR-BII (NP_005497.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Rabbit (100%); Monkey, Mouse, Hamster, Elephant, Panda, Pig (93%); Rat, Bovine, Dog, Bat (87%); Horse (80%).
Blocking Peptide
LS-E27038 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8307. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa96-110
Specificity
Human SCARB2 / SR-BII.
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx. 80kDa band observed in Human Brain (Cortex and Cerebellum) lysates (calculated MW of 54.3kDa according to NP_005497.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fujita et al, Biochem Biophys Res Commun. 1992 Apr 30;184(2):604-11.; PMID: 1374238). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-LIMPII / SCARB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8307 dilution 3.75 ug/ml.
Anti-LIMPII / SCARB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8307 dilution 3.75 ug/ml.

Western blot

SCARB2 antibody (0.1 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SCARB2 antibody (0.1 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-LIMPII / SCARB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8307 dilution 3.75 ug/ml.
Anti-LIMPII / SCARB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8307 dilution 3.75 ug/ml.

Western blot

SCARB2 antibody (0.1 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SCARB2 antibody (0.1 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-LIMPII / SCARB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8307 dilution 3.75 ug/ml.
Anti-LIMPII / SCARB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8307 dilution 3.75 ug/ml.

Western blot

SCARB2 antibody (0.1 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SCARB2 antibody (0.1 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-LIMPII / SCARB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8307 dilution 3.75 ug/ml.
Anti-LIMPII / SCARB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8307 dilution 3.75 ug/ml.

Western blot

SCARB2 antibody (0.1 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SCARB2 antibody (0.1 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 7/17/2018

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