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Anti-LIMK2 Antibody (aa294-655) LS-C185916

Catalog Size Price
LS-C185916-100 100 µl (1 mg/ml) Unavailable

Related Products

40 LIMK2 Antibodies

Most Popular LIMK2 Antibodies

Anti-LIMK2 Antibody (aa249-298) LS-C118289
Rabbit Polyclonal (IgG) to Human LIMK2
Human, Mouse, Rat
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Western blot of extracts from NIH-3T3 cells, treated with PMA 125 ng/ml 30', using LIMK2 Antibody. The lane on the right is treated with the synthesized peptide.
Anti-LIMK2 Antibody (aa461-510) LS-C118940
Rabbit Polyclonal (IgG) to Human LIMK2
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunofluorescence of HeLa cells, using LIMK2 Antibody. The picture on the right is treated with the synthesized peptide.
Anti-LIMK2 Antibody (Internal) LS-C352456
Rabbit Polyclonal to Human LIMK2
Human
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of LIMK2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-LIMK2 Antibody (phospho-Thr505) LS-C380758
Rabbit Polyclonal (IgG) to Human LIMK2
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot of Phospho-LIMK-2 (T505) antibody
Anti-LIMK2 Antibody (aa430-510) LS-C384348
Rabbit Polyclonal (IgG) to Human LIMK2
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot of LIMK-2 antibody

100% Guaranteed
Rabbit Polyclonal (IgG) to Human LIMK2
Human
IHC, IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human LIMK2
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

LIMK2 antibody was raised against recombinant protein fragment contain a sequence corresponding to a region within amino acids 294 and 655 of LIM kinase 2.
aa294-655
Human LIMK2
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About LIMK2

P53671 NM_016733 NP_057952.1

LIMK2 Antibody, LIM domain kinase 2 Antibody, LIM kinase 2 Antibody, LIMK-2 Antibody

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain.

Immunohistochemistry

IHC of paraffin-embedded CA922 xenograft using LIM kinase 2 antibody at 1:500 dilution.
IHC of paraffin-embedded CA922 xenograft using LIM kinase 2 antibody at 1:500 dilution.

Immunocytochemistry

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using LIM kinase 2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using LIM kinase 2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Western blot

Sample (30g whole cell lysate). A:293T. 7.5% SDS PAGE. LIMK2 antibody diluted at 1:1000
Sample (30g whole cell lysate). A:293T. 7.5% SDS PAGE. LIMK2 antibody diluted at 1:1000

Requested From: 
Date Requested: 4/22/2018

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