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Anti-LIMK1 / LIMK Antibody (clone 6A7) LS-C172994

Catalog Size Price
LS-C172994-100 100 µl (1 mg/ml) Unavailable

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69 LIMK1 / LIMK Antibodies

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100% Guaranteed
Mouse Monoclonal [clone 6A7] (IgG2a) to Human LIMK1 / LIMK
Human
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human LIMK1 / LIMK
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [6A7]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

LIMK1 / LIMK antibody was raised against full length human recombinant protein of human LIMK1(NP_002305) produced in HEK293 cell.
Human LIMK1 / LIMK

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About LIMK1 / LIMK

P53667 NM_002314 NP_002305.1

LIMK1 Antibody, LIM domain kinase 1 Antibody, Lim kinase 1 Antibody, LIMK-1 Antibody, LIM kinase Antibody, LIMK Antibody

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop.

Immunofluorescence

Immunofluorescence
Anti-LIMK1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIMK1.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIMK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMK1.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIMK1 antibody, and then analyzed by flow cytometry.

Requested From: 
Date Requested: 12/12/2017

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