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Anti-LIMK1 / LIMK Antibody (clone 1A8) IHC-plus™ LS-B5430

Note: This antibody replaces LS-C133293

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $450

LSBio (Direct) LSBio (Direct)
206-374-1102
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LIMK1 / LIMK Antibody LS-B5430 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-LIMK1 antibody IHC of human small intestine.
Western blot
LIMK1 monoclonal antibody clone 1A8. Western blot of LIMK1 expression in PC-12.
Western blot
LIMK1 monoclonal antibody clone 1A8. Western blot of LIMK1 expression in NIH/3T3.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged LIMK1 is approximately 0.03 ng/ml as a capture antibody.

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Mouse Monoclonal [clone 1A8] (IgG2a,k) to Human LIMK1 / LIMK
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human LIMK1 / LIMK
Mouse
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [1A8]
Protein A purified

Applications

IHC - Paraffin (10 µg/ml), Western blot, ELISA

Specificity and Use

LIMK1 / LIMK antibody was raised against lIMK1 (NP_002305, 548 a.a. ~ 648 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human LIMK1
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell lysates and the recombinant protein used as the immunogen.

Packaging

PBS, pH 7.4.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About LIMK1 / LIMK

P53667 NM_002314 NP_002305.1

LIMK1 Antibody, LIM domain kinase 1 Antibody, Lim kinase 1 Antibody, LIMK-1 Antibody, LIM kinase Antibody, LIMK Antibody

LIMK1, a LIMK -type protein kinase, is a component of the cellular motility machinery regulating actin filament dynamics. The protein is phosphorylated by the coiled-coil-containing protein kinase ROCK, a downstream effector of the GTPase Rho. Activated LIMK1 phosphorylates cofilin and thereby inhibits its actin-depolymerizing activity. At least two protein isoforms of 305 and 647 amino acids have been reported.

Immunohistochemistry

Immunohistochemistry
Anti-LIMK1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5430 concentration 10 ug/ml.

Western blot

Western blot
LIMK1 monoclonal antibody clone 1A8. Western blot of LIMK1 expression in PC-12.

Western blot

Western blot
LIMK1 monoclonal antibody clone 1A8. Western blot of LIMK1 expression in NIH/3T3.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged LIMK1 is approximately 0.03 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 4/25/2015

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