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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LIME1 / LIME Antibody (aa258‑269) LS‑C20175
Note: This antibody replaces LS-C71570
LIME antibody LS-C20175 is an unconjugated goat polyclonal antibody to LIME (LIME1) from mouse and rat. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C20175-100
100 µg (0.5 mg/ml)
$405
Description
LIME antibody LS-C20175 is an unconjugated goat polyclonal antibody to LIME (LIME1) from mouse and rat. Validated for Peptide-ELISA and WB.
Target
Mouse LIME1 / LIME
Host
Goat
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Immunogen
LIME1 / LIME antibody was raised against synthetic peptide C-ENVYESIKEMGL from the C-terminus of mouse LIME1 / LIME (NP_076173.1). Percent identity by BLAST analysis: Mouse, Rat (100%); Bovine, Bat, Horse (83%).
Blocking Peptide
LS-E25731 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C20175. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa258-269
Specificity
Mouse LIME1 / LIME.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About LIME1 / LIME
Q9H400 NM_017806 NP_060276.2

Popular LIME1 / LIME Products

Western blot of  mouse thymocyte lysate (1% SDS); non-reduced sample, immunostained by mAb mLIME-05 and goat anti-mouse IgG (H+L)-HRP conjugate.
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IHC of paraffin-embedded Human Kidney tissue using anti-LIME1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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Antibody
Species: Mouse
Applications: Western blot, Immunoprecipitation

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Images

Western blot

Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/16/2018
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