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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LIME1 / LIME Antibody (aa258‑269) LS‑C20175
Note: This antibody replaces LS-C71570
LIME antibody LS-C20175 is an unconjugated goat polyclonal antibody to LIME (LIME1) from mouse and rat. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C20175-100
100 µg (0.5 mg/ml)
$405

Popular LIME1 / LIME Products

Anti-LIME1 / LIME antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1878 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-LIME1 / LIME antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3731 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Mouse
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
IHC of paraffin-embedded Human Kidney tissue using anti-LIME1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Western blot of  mouse thymocyte lysate (1% SDS); non-reduced sample, immunostained by mAb mLIME-05 and goat anti-mouse IgG (H+L)-HRP conjugate.
Species: Mouse
Applications: Western blot, Immunoprecipitation

Product Description

LIME antibody LS-C20175 is an unconjugated goat polyclonal antibody to LIME (LIME1) from mouse and rat. Validated for Peptide-ELISA and WB.
About LIME1 / LIME
Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules. Q9H400 NM_017806 NP_060276.2

LIME1 Antibody, DJ583P15.4 Antibody, Lck-interacting molecule Antibody, LIME Antibody, RP4-583P15.5 Antibody, LP8067 Antibody


Specifications

Target
Mouse LIME1 / LIME
Host
Goat
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Immunogen
LIME1 / LIME antibody was raised against synthetic peptide C-ENVYESIKEMGL from the C-terminus of mouse LIME1 / LIME (NP_076173.1). Percent identity by BLAST analysis: Mouse, Rat (100%); Bovine, Bat, Horse (83%).
Blocking Peptide
LS-E25731 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C20175. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa258-269
Specificity
Mouse LIME1 / LIME.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Western blot

Staining (1?g/ml) of Mouse Heart lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Staining (1?g/ml) of Mouse Heart lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot

Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Staining (1?g/ml) of Mouse Heart lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Staining (1?g/ml) of Mouse Heart lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot

Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Staining (1?g/ml) of Mouse Heart lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Staining (1?g/ml) of Mouse Heart lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot

Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Staining (1?g/ml) of Mouse Heart lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Staining (1?g/ml) of Mouse Heart lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot

Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (1 ug/ml) of Mouse Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 9/19/2018

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