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Anti-LIG4 / DNA Ligase IV Antibody (aa275-324) LS-C31455

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl 1 mg/ml $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular LIG4 / DNA Ligase IV Antibodies

Anti-LIG4 / DNA Ligase IV Antibody (aa275-324) LS-C31455
Rabbit Polyclonal (IgG) to Human LIG4 / DNA Ligase IV
Human, Monkey, Horse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-LIG4 / DNA Ligase IV Antibody (aa275-324) LS-C121177
Rabbit Polyclonal to Human LIG4 / DNA Ligase IV
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-LIG4 / DNA Ligase IV Antibody LS-C331665
Rabbit Polyclonal (IgG) to Human LIG4 / DNA Ligase IV
Human, Mouse, Rat
IHC, Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human LIG4 / DNA Ligase IV
Human, Monkey, Horse
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human LIG4 / DNA Ligase IV
Rabbit
Human, Monkey, Horse (tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Protein A purified
Unmodified

Applications

  • IHC - Paraffin
  • Western blot (1.25 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

LIG4 / DNA Ligase IV antibody was raised against synthetic peptide located between aa275-324 of human LIG4 (Q8IY66, NP_002303). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%); Galago, Hamster, Panda, Dog, Bovine, Rabbit, Pig, Guinea pig (92%); Mouse, Rat (91%); Elephant, Bat, Horse, Opossum, Turkey, Zebra finch, Chicken, Lizard, Xenopus (85%).
aa275-324
Human LIG4

Packaging

Lyophilized from PBS with 2% sucrose
100 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About LIG4 / DNA Ligase IV

P49917 NM_002312 NP_002303.2

LIG4 Antibody, DNA ligase IV Antibody, DNA joinase Antibody, DNA ligase 4 Antibody, DNA repair enzyme Antibody, Ligase IV, DNA, ATP-dependent Antibody, Sealase Antibody, Polynucleotide ligase Antibody

LIG4 / DNA Ligase IV is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ.

Immunohistochemistry

Immunohistochemistry
LIG4 antibody LS-C31455 was used in IHC to stain formalin-fixed, paraffin-embedded human pancreas. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/4/2016

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