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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1698-50 | 50 µl (1 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human LIG1 / DNA Ligase 1 Antibody (clone 10H5, IHC, WB) LS‑B1698
IHC‑plus™ Monoclonal Mouse anti‑Human LIG1 / DNA Ligase 1 Antibody (clone 10H5, IHC, WB) LS‑B1698
Note: This antibody replaces LS-C19958
Antibody:
LIG1 / DNA Ligase 1 Mouse anti-Human Monoclonal (10H5) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
LIG1 / DNA Ligase 1 Mouse anti-Human Monoclonal (10H5) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
DNA Ligase 1 antibody LS-B1698 is an unconjugated mouse monoclonal antibody to human DNA Ligase 1 (LIG1). Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human LIG1 / DNA Ligase 1
Synonyms
LIG1 | DNA ligase I | DNA ligase 1 | Ligase I, DNA, ATP-dependent
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
10H5
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Full-length recombinant human DNA Ligase I protein
Specificity
Full-length recombinant human DNA Ligase I protein
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1698 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1698 was determined to be 5 ug/ml.
Presentation
PBS
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About LIG1 / DNA Ligase 1
Validation
Anti-LIG1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-LIG1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ LIG1 / DNA Ligase 1 Antibody (clone 10H5) for IHC, WB/Western, IP LS-B1698 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Monkey, Rat, Hamster
Applications:
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse
Applications:
IHC, Immunofluorescence, Western blot
Source:
E. coli
Tag:
His, N-Terminal
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
