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Anti-LIG1 / DNA Ligase 1 Antibody (clone 10H5) IHC-plus™ LS-B1698

Note: This antibody replaces LS-C19958

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular LIG1 / DNA Ligase 1 Antibodies

Anti-LIG1 / DNA Ligase 1 Antibody (aa1-919) LS-C83542
Mouse Monoclonal (IgG1) to Human LIG1 / DNA Ligase 1
Human
Western blot, Immunoprecipitation
Unconjugated
Anti-LIG1 / DNA Ligase 1 Antibody (clone 10G12) IHC-plus™ LS-B6111
Mouse Monoclonal [clone 10G12] (IgG2a,k) to Human LIG1 / DNA Ligase 1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-LIG1 / DNA Ligase 1 Antibody (clone 5H5) LS-C179717
Mouse Monoclonal [clone 5H5] (IgG1) to Human LIG1 / DNA Ligase 1
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 10H5] (IgG1) to Human LIG1 / DNA Ligase 1
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human LIG1 / DNA Ligase 1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [10H5]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • Immunoprecipitation

Specificity and Use

LIG1 / DNA Ligase 1 antibody was raised against recombinant human LIG1.
Full-length recombinant human DNA Ligase I protein
Immunohistochemistry: LS-B1698 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1698 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.2. No preservatives added.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About LIG1 / DNA Ligase 1

P18858 NM_000234 NP_000225.1

LIG1 Antibody, DNA ligase I Antibody, DNA ligase 1 Antibody, Ligase I, DNA, ATP-dependent Antibody

LIG1 / DNA Ligase 1 is a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.

Immunohistochemistry

Immunohistochemistry
Anti-LIG1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1698 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/7/2016

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