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Catalog Number Size Price
LS-C112847-100 100 µg (0.5 mg/ml) $503 
LIG1 / DNA Ligase 1 Antibody - LIG1 antibody (0.1 ug/ml) staining of HeLa nuclear lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Polyclonal Goat anti‑Human LIG1 / DNA Ligase 1 Antibody (aa877‑890, WB) LS‑C112847

Polyclonal Goat anti‑Human LIG1 / DNA Ligase 1 Antibody (aa877‑890, WB) LS‑C112847

Antibody:
LIG1 / DNA Ligase 1 Goat anti-Human Polyclonal (aa877-890) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rat, Hamster
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C112847-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
LIG1 / DNA Ligase 1 Goat anti-Human Polyclonal (aa877-890) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rat, Hamster
Format:
Unconjugated, Unmodified

Specifications

Description
DNA Ligase 1 antibody LS-C112847 is an unconjugated goat polyclonal antibody to DNA Ligase 1 (LIG1) (aa877-890) from human. It is reactive with human, rat, hamster and other species. Validated for Peptide-ELISA and WB.
Target
Human LIG1 / DNA Ligase 1
Synonyms
LIG1 | DNA ligase I | DNA ligase 1 | Ligase I, DNA, ATP-dependent
Host
Goat
Reactivity
Human, Monkey, Rat, Hamster (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-RVREDKQPEQATTS, from the internal region (near C-Terminus) of the protein sequence according to NP_000225.1.
Epitope
aa877-890
Specificity
Human LIG1.
Applications
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LIG1 / DNA Ligase 1
P18858 NM_000234 NP_000225.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024