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Anti-LIG1 / DNA Ligase 1 Antibody (aa877-890) LS-C112847

Catalog Size Price
LS-C112847-100 100 µg (0.5 mg/ml) Unavailable

Most Popular LIG1 / DNA Ligase 1 Antibodies

Anti-LIG1 / DNA Ligase 1 Antibody (clone 10H5) IHC-plus™ LS-B1698
Mouse Monoclonal [clone 10H5] (IgG1) to Human LIG1 / DNA Ligase 1
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-LIG1 / DNA Ligase 1 Antibody (aa1-919) LS-C83542
Mouse Monoclonal (IgG1) to Human LIG1 / DNA Ligase 1
Human
Western blot, Immunoprecipitation
Unconjugated
Anti-LIG1 / DNA Ligase 1 Antibody (aa877-890) LS-C112847
Goat Polyclonal to Human LIG1 / DNA Ligase 1
Human, Monkey, Rat, Hamster
Western blot, ELISA
Unconjugated
Western blot Image
Anti-LIG1 / DNA Ligase 1 Antibody (clone 10G12) IHC-plus™ LS-B6111
Mouse Monoclonal [clone 10G12] (IgG2a,k) to Human LIG1 / DNA Ligase 1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-LIG1 / DNA Ligase 1 Antibody LS-C331736
Rabbit Polyclonal (IgG) to Human LIG1 / DNA Ligase 1
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image

100% Guaranteed
Goat Polyclonal to Human LIG1 / DNA Ligase 1
Human, Monkey, Rat, Hamster
Western blot, ELISA
Unconjugated

Details

Human LIG1 / DNA Ligase 1
Goat
Human, Monkey, Rat, Hamster (tested or 100% immunogen sequence identity)
Mouse, Bat, Bovine (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.3 µg/ml)
  • ELISA (1:16000)

Specificity and Use

Synthetic peptide C-RVREDKQPEQATTS from an internal region (near C-terminus) of human LIG1 (NP_000225.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Rat, Hamster (100%); Mouse, Bat, Bovine, Toxoplasma, Neospora (93%); Dog, Rice (86%).
LS-E27117 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C112847. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa877-890
Human LIG1.
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About LIG1 / DNA Ligase 1

P18858 NM_000234 NP_000225.1

LIG1 Antibody, DNA ligase I Antibody, DNA ligase 1 Antibody, Ligase I, DNA, ATP-dependent Antibody

LIG1 / DNA Ligase 1 is a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.

Western blot

Western blot
LIG1 antibody (0.1 ug/ml) staining of HeLa nuclear lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 12/12/2017

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