Frog, Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- IHC - Paraffin (1:200 - 1:1000)
- ICC (1:500)
- Western blot (1:3000 - 1:6000)
- Immunoprecipitation (1:200)
- (applications tested for the base form of this product only)
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LHX1 antibody was raised against c-terminal portion of frog LIM-1 protein.
Recognizes frog LIM-1. Does not appear to cross-react with LIM-5 (the closest homolog of LIM-1 in frog) on tissue sections, but does cross-react with LIM-5 in Western Blot and Immunoprecipitation. Species cross-reactivity: human, rat, mouse and fish. Also reacts with LIM-1 protein from whole-mount fish embryo.
Suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry and Immunoprecipitation. Not suitable for Immunofluorescence. Immunohistochemistry (paraffin embedded sections): 1:200-1:1000 (Fish and whole mounts: 1:500) Recommended fixative: MEMFA. Western Blot: 1:3000-1:6000. Immunocytochemistry: 1:500 on P19 cell line. Immunoprecipitation: 1:200.
0.02M PBS, pH 7.6, 0.1% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.