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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LHX1 Antibody (C‑Terminus) LS‑C16214
LHX1 antibody LS-C16214 is an unconjugated rabbit polyclonal antibody to LHX1 from human, mouse, rat and other species. Validated for ICC, IHC, IP and WB.
100 µg (1 mg/ml)

Popular LHX1 Products

Anti-LHX1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1574 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Anti-LHX1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5429 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-LHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Immunofluorescent analysis of LHX1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot

Product Description

LHX1 antibody LS-C16214 is an unconjugated rabbit polyclonal antibody to LHX1 from human, mouse, rat and other species. Validated for ICC, IHC, IP and WB.


Frog LHX1
LHX1, Homeobox protein Lim-1, LIM1, HLim-1, LIM homeobox 1, LIM homeobox protein 1, LIM-1, LIM/homeobox protein Lhx1
Frog, Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Protein A purified
  • IHC
  • IHC - Paraffin (1:200 - 1:1000)
  • ICC (1:500)
  • Western blot (1:3000 - 1:6000)
  • Immunoprecipitation (1:200)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
LHX1 antibody was raised against c-terminal portion of frog LIM-1 protein.
Recognizes frog LIM-1. Does not appear to cross-react with LIM-5 (the closest homolog of LIM-1 in frog) on tissue sections, but does cross-react with LIM-5 in Western Blot and Immunoprecipitation. Species cross-reactivity: human, rat, mouse and fish. Also reacts with LIM-1 protein from whole-mount fish embryo.
Suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry and Immunoprecipitation. Not suitable for Immunofluorescence. Immunohistochemistry (paraffin embedded sections): 1:200-1:1000 (Fish and whole mounts: 1:500) Recommended fixative: MEMFA. Western Blot: 1:3000-1:6000. Immunocytochemistry: 1:500 on P19 cell line. Immunoprecipitation: 1:200.
0.02M PBS, pH 7.6, 0.1% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
About LHX1
LHX1 is a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract. P48742 NM_005568 NP_005559.2

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Requested From: United States
Date Requested: 10/21/2018

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