Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
LHX1 antibody was raised against c-terminal portion of frog LIM-1 protein.
Recognizes frog LIM-1. Does not appear to cross-react with LIM-5 (the closest homolog of LIM-1 in frog) on tissue sections, but does cross-react with LIM-5 in Western Blot and Immunoprecipitation. Species cross-reactivity: human, rat, mouse and fish. Also reacts with LIM-1 protein from whole-mount fish embryo.
Suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry and Immunoprecipitation. Not suitable for Immunofluorescence. Immunohistochemistry (paraffin embedded sections): 1:200-1:1000 (Fish and whole mounts: 1:500) Recommended fixative: MEMFA. Western Blot: 1:3000-1:6000. Immunocytochemistry: 1:500 on P19 cell line. Immunoprecipitation: 1:200.
0.02M PBS, pH 7.6, 0.1% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
LHX1 is a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract.