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Anti-LHX1 Antibody (C-Terminus) LS-C16214

Catalog Size Price
LS-C16214-100 100 µg (1 mg/ml) Unavailable

Related Products

34 LHX1 Antibodies

Most Popular LHX1 Antibodies

Anti-LHX1 Antibody (C-Terminus) LS-C16214
Rabbit Polyclonal (IgG) to Frog LHX1
Frog, Human, Mouse, Rat
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Anti-LHX1 Antibody (aa120-169) IHC-plus™ LS-B1574
Rabbit Polyclonal (IgG) to Human LHX1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Chicken
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-LHX1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1574 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-LHX1 Antibody (clone 2G10) IHC-plus™ LS-B5429
Mouse Monoclonal [clone 2G10] (IgG2b,k) to Human LHX1
Human
IHC, IHC - Paraffin, ELISA
Unconjugated
Anti-LHX1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5429 concentration 5 ug/ml.
Anti-LHX1 Antibody (aa100-362, clone 2D5) LS-C337681
Mouse Monoclonal [clone 2D5] (IgG1) to Human LHX1
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-LHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Anti-LHX1 Antibody (Internal) LS-C352449
Rabbit Polyclonal to Human LHX1
Human, Mouse, Rat
ICC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescent analysis of LHX1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

100% Guaranteed
Rabbit Polyclonal (IgG) to Frog LHX1
Frog, Human, Mouse, Rat
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation (applications tested for the base form of this product only)
Unconjugated

Details

Frog LHX1
Rabbit
Frog, Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Protein A purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:200 - 1:1000)
  • ICC (1:500)
  • Western blot (1:3000 - 1:6000)
  • Immunoprecipitation (1:200)
  • (applications tested for the base form of this product only)

Specificity and Use

LHX1 antibody was raised against c-terminal portion of frog LIM-1 protein.
C-Terminus
Recognizes frog LIM-1. Does not appear to cross-react with LIM-5 (the closest homolog of LIM-1 in frog) on tissue sections, but does cross-react with LIM-5 in Western Blot and Immunoprecipitation. Species cross-reactivity: human, rat, mouse and fish. Also reacts with LIM-1 protein from whole-mount fish embryo.
Suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry and Immunoprecipitation. Not suitable for Immunofluorescence. Immunohistochemistry (paraffin embedded sections): 1:200-1:1000 (Fish and whole mounts: 1:500) Recommended fixative: MEMFA. Western Blot: 1:3000-1:6000. Immunocytochemistry: 1:500 on P19 cell line. Immunoprecipitation: 1:200.

Packaging

0.02M PBS, pH 7.6, 0.1% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About LHX1

P48742 NM_005568 NP_005559.2

LHX1 Antibody, Homeobox protein Lim-1 Antibody, LIM1 Antibody, HLim-1 Antibody, LIM homeobox 1 Antibody, LIM homeobox protein 1 Antibody, LIM-1 Antibody, LIM/homeobox protein Lhx1 Antibody

LHX1 is a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract.

Requested From: 
Date Requested: 5/20/2018

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