Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone M3/38] (IgG2a,k) to Mouse LGALS3 / Galectin 3
Mouse LGALS3 / Galectin 3
Mouse (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [M3/38]
Specificity and Use
This M3/38 (M3/38) antibody has been tested by intracellular staining and flow cytometric analysis. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Staining can be done using Foxp3 buffers or IC Fixation Buffers. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
LGALS3 / Galectin 3 is a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands.
Intracellular staining of the monocyte population in human PBMCs or C57 mouse bone marrow cells (left) with APC anti-mouse CD11b (M1/70) (LS-C107298) and 0.125 ug of Biotin anti-mouse Galectin-3 (M3/38)followed by SAv-PE. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Intracellular staining of the monocyte population in human PBMCs (right) or C57 mouse bone marrow cells followed by SAv-PE. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.