Human LF / LTF / Lactoferrin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

1A1

Unconjugated

Affinity purified

Unmodified

Purified lactoferrin from human milk.

Recognizes human lactoferrin, a 78kD globular protein found in body secretions such as milk and saliva and is a member of the transferrin family proteins. Transferrins control the level of free iron in the body, by binding and transporting iron. Lactoferrin does not appear to have a transporting function, instead it has antimicrobial activity, by keeping the levels of iron in body fluids low and thus preventing microbes from acquiring iron. Removing free iron from body fluids and inflamed areas also serve to protect against the damaging effects of free radicals. Lactoferrin is used as a therapeutic and prophylactic treatment of various illnesses, such as cancers and intestinal illnesses. May be used to detect lactoferrin in ELISA assays.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot
• ELISA

Immunohistochemistry: LS-B2636 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2636 was determined to be 10 ug/ml. ELISA: May be used in an indirect ELISA or as a detection antibody in a sandwich ELISA together with LS-C58299 as a capture agent.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02788 NCBI: NM_002343 NP_002334.2